fcmedicineФундаментальная и клиническая медицинаFundamental and Clinical Medicine2500-07642542-0941КемГМУ10.23946/2500-0764-2019-4-3-8-14fcmedicine-154Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL RESEARCHПолиморфизм генов цитохромов у женщин с синдромом поликистозных яичниковPolymorphisms of cytochrome genes in women with polycystic ovary syndromehttps://orcid.org/0000-0001-5574-4275БегловаА. Ю.BeglovaA. Yu.

ассистент кафедры акушерства и гинекологии имени профессора Г. А. Ушаковой,

22а, ул. Ворошилова, г. Кемерово, 650056 

MD, Assistant Professor, Department of Obstetrics and Gynecology,

22a, Voroshilova Street, Kemerovo, 650056

angelik-1986@mail.ruЕлгинаС. И.YelginaS. I.

профессор кафедры акушерства и гинекологии имени профессора Г. А. Ушаковой, доцент, доктор медицинских наук,

Кемерово

MD, DSc, Professor, Department of Obstetrics and Gynecology,

Kemerovo

https://orcid.org/0000-0001-7014-6492АртымукН. В.ArtymukN. V.

доктор медицинских наук, профессор, заведующая кафедрой акушерства и гинекологии имени профессора Г. А. Ушаковой,

Кемерово

MD, DSc, Head of the Department of Obstetrics and Gynecology,

Kemerovo

https://orcid.org/0000-0002-2050-1342ГордееваЛ. А.GordeevaL. A.

доктор медицинских наук, ведущий научный сотрудник лаборатории иммуногенетики Института экологии человека,

Кемерово

MD, PhD, Leading Researcher, Laboratory of Immunogenetics, 

18, Sovetskiy Prospekt, Kemerovo, 650000

ФГБОУ ВО «Кемеровский государственный медицинский университет» Министерства здравоохранения Российской ФедерацииРоссияKemerovo State Medical UniversityRussian FederationФГБНУ «Федеральный исследовательский центр угля и углехимии СО РАН»РоссияInstitute of Human Ecology, Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of SciencesRussian Federation20193009201943814Copyright © Беглова А.Ю., Елгина С.И., Артымук Н.В., Гордеева Л.А., 20192019Беглова А.Ю., Елгина С.И., Артымук Н.В., Гордеева Л.А.Beglova A.Y., Yelgina S.I., Artymuk N.V., Gordeeva L.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fcm.kemsmu.ru/jour/article/view/154Цель

Цель. Изучение полиморфизма генов CYP11А1, CYP17A1, CYP19A1 у женщин с синдромом поликистозных яичников (СПКЯ) репродуктивного возраста в сравнении с женщинами без СПКЯ.

Материалы и методы

Материалы и методы. В исследование включено 188 женщин репродуктивного возраста. I группу составили 94 женщин с СПКЯ; II группу – 94 женщины без СПКЯ. Всем пациенткам проведен молекулярно-генетический анализ полиморфизмов генов VNTR пентануклеотидного ((tttta)n) полиморфизма в позиции -528 промоторного региона гена CYP11A1 и CYP17A1(-34T>C (MspA1), rs743572) и CYP19A1 (c.-39+15658 C>T, C40824T, rs2470152) с использованием тест-системы ООО «СибДНК» (г. Новосибирск), с последующей статистической обработкой данных.

Результаты

Результаты. Распределение частот генотипов генов CYP11A1 (tttta)n, CYP17A1 rs743572 и CYP19A1 rs2470152 в группах женщин с СПКЯ и у здоровых женщин статистически значимо не отличалось (р>0,05). Однако для полиморфизма CYP11A1 (tttta)n наблюдалась тенденция к накоплению аллелей с большим количеством (tttta)n-повторов у женщин с СПКЯ, чем у здоровых женщин. Типичны были VNTR генотипы с 6/6, 6/8 и 8/8 пентануклеотидными повторами.

Заключение

Заключение. Полиморфизм исследованных генов цитохромов не играл ключевой роли в развитии СПКЯ в обследованной группе женщин. Наше исследование может быть полезным для проведения последующих мета-анализов, которые могут позволить раскрыть представление о патогенезе заболевания. 

Aim

Aim. To study the polymorphisms of CYP11A1, CYP17A1, and CYP19A1 genes in women with polycystic ovary syndrome (PCOS) of reproductive age in comparison with women without PCOS.

Materials and Methods

Materials and Methods. We consecutively recruited 94 women with polycystic ovary syndrome of reproductive age and 94 age-matched healthy women. All patients were subjected to molecular genetic analysis of pentanucleotide ((tttta)n) polymorphism at -528 position within the promoter region of the CYP11A1 gene, rs743572 polymorphism within the CYP17A1 gene, and rs2470152 polymorphism within the CYP19A1 gene. We further compared the frequencies of respective genotypes and alleles between the groups.

Results

Results. Genotype distribution of the indicated polymorphisms did not differ significantly between the groups. However, for CYP11A1 (tttta)n polymorphism, a trend to the accumulation of the alleles with a large number of (tttta)n-repeats was observed in women with polycystic ovary syndrome as compared to healthy women. The most common were VNTR genotypes with 6/6, 6/8 and 8/8 pentanucleotide repeats.

Conclusion

Conclusion. The studied polymorphisms of cytochrome genes did not affect the risk of PCOS in the examined group of women. Our study may be useful for the further meta-analyses related to the genetic predisposition to PCOS. 

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The authors declare that there are no conflicts of interest present.