fcmedicineФундаментальная и клиническая медицинаFundamental and Clinical Medicine2500-07642542-0941КемГМУ10.23946/2500-0764-2020-5-3-24-34fcmedicine-295Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL RESEARCHЭффективность гетерологического деминерализованного костного матрикса для замещения критических костных дефектов свода черепа крысHeterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defectshttps://orcid.org/0000-0001-9946-1015ВеремеевА. В.VeremeevA. V.

Веремеев Алексей Владимирович, кандидат медицинских наук, генеральный директор

125252, г. Москва, ул. Авиаконструктора Микояна, д. 12, корп. А, п. 1, эт. 2, оф. 1

Dr. Alexey V. Veremeev, MD, PhD, Chief Executive Officer

125252, Aviakonstruktora Mikoyana Street, 12, А, 2nd Floor, Office 1, Moscow

al.veremeev@gmail.comhttps://orcid.org/0000-0001-8679-4857БолгаринР. Н.BolgarinR. N.

Болгарин Роман Николаевич, директор по развитию

125252, Москва, ул. Авиаконструктора Микояна, д. 12, корп. А, п. 1, эт. 2, оф. 1

Mr. Roman N. Bolgarin, Development Director

125252, Aviakonstruktora Mikoyana Street, 12, А, 2nd Floor, Office 1, Moscow

https://orcid.org/0000-0001-5623-2466НестеренкоВ. Г.NesterenkoV. G.

Нестеренко Владимир Георгиевич, доктор медицинских наук, профессор, заведующий отделом иммунологии

123098, г. Москва, ул. Гамалеи, д. 18

Prof. Vladimir G. Nesterenko, MD, DSc, Professor, Head of the Immunology Department

123098, Gamaleya Street, 18, Moscow

https://orcid.org/0000-0002-1173-8153Андреев-АндриевскийА. А.Andreev-AndrievskiyA. A.

Андреев-Андриевский Александр Александрович, кандидат биологических наук, руководитель центра доклинических исследований ООО «НИИ митоинженерии МГУ»

119330, г. Москва, ул. Ленинские горы, д. 73А

Dr. Alexander A. Andreev-Andrievskiy, PhD, Head of the Center for Preclinical Trials, Mitoengineering Research Institute LLC

119330, Leninskie Gory Street, 73A, Moscow

Общество с ограниченной ответственностью «Матрифлекс»; ФГБУ «Национальный исследовательский центр пидемиологии и микробиологии имени почётного академика Н. Ф. Гамалеи» Министерства здравоохранения Российской ФедерацииРоссияMatriflex LLC; Gamaleya National Research Centre of Epidemiology and MicrobiologyRussian FederationОбщество с ограниченной ответственностью «Матрифлекс»РоссияMatriflex LLCRussian FederationФГБУ «Национальный исследовательский центр эпидемиологии и микробиологии имени почётного академика Н. Ф. Гамалеи» Министерства здравоохранения Российской ФедерацииРоссияGamaleya National Research Centre of Epidemiology and MicrobiologyRussian FederationМосковский государственный университет имени М.В. ЛомоносоваРоссияMoscow State UniversityRussian Federation202029092020532434Copyright © Веремеев А.В., Болгарин Р.Н., Нестеренко В.Г., Андреев-Андриевский А.А., 20202020Веремеев А.В., Болгарин Р.Н., Нестеренко В.Г., Андреев-Андриевский А.А.Veremeev A.V., Bolgarin R.N., Nesterenko V.G., Andreev-Andrievskiy A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fcm.kemsmu.ru/jour/article/view/295

Цель. Оценить эффективность гетерологического деминерализованного костного матрикса (ДКМ) для замещения костных дефектов в эксперименте на модели критического дефекта костей свода черепа крыс. Материалы и методы. Для эксперимента использовали самцов крыс (n = 48) линии Sprague-Dawley возрастом от 4,5 до 6 месяцев. В ходе оперативного вмешательства создавали критический дефект костей свода черепа и замещали его костным аутотрансплантатом, гетерологическим ДКМ или препаратом-компаратором (Geistlich Bio-Oss®) либо оставляли незаполненным (отрицательный контроль). Через 4 или 12 недель выводили крыс из эксперимента и проводили исследование областей дефекта при помощи микроскопической оценки срезов тканей, окрашенных гематоксилином и эозином (доля минерализованной ткани от просвета дефекта) и микрокомпьютерной томографии (объем новообразованной костной ткани, минеральная плотность новообразованной ткани, толщина новообразованных костных элементов и распределение их диаметра). Результаты. Замещение дефектной области костным аутотрансплантатом, как и ожидалось, показало наилучшие результаты. При применении гетерологического ДКМ и препарата-компаратора наблюдалась выраженное заполнение критического дефекта, при этом статистически значимых различий в показателях репарации костной ткани между разработанным оригинальным прототипом и медицинским изделием сравнения выявлено не было. Микрокомпьютерно-томографический и гистологический методы исследования продемонстрировали конкордантные результаты (эффекты имели сопоставимую степень выраженности). Заключение. Гетерологический ДКМ эффективен для замещения критических костных дефектов свода черепа крыс.

Aim. To evaluate the efficacy of heterologous demineralised bone matrix (DBM) for the replacement of bone defects using a critical-sized rat calvarial defect model. Materials and Methods. For the experiments, we used 48 Sprague-Dawley rats (4.5 to 6 months of age). Critical-sized (8 mm diameter) calvarial defect was filled by the bone autograft, heterologous DBM, or comparator product (Geistlich BioOss®) or remained unfilled (negative control). Upon 4 or 12 weeks, rats were euthanised with the subsequent investigation of the defect and adjacent tissues by means of hematoxylin and eosin staining (mineralized tissue area to the defect area ratio) and microcomputed tomography (volume, thickness, and mineral density of the repaired tissue). Results. In our experimental setting, bone autograft was the most efficient in bone repair. Heterologous DBM and comparator product were equally efficient in filling the defect and did not show any statistically significant differences regarding any of the parameters. Microcomputed tomography and routine histological examination demonstrated concordant results. Conclusion. Heterologous DBM is efficient for the repair of critical-sized rat calvarial defects.

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The authors declare that there are no conflicts of interest present.