fcmedicineФундаментальная и клиническая медицинаFundamental and Clinical Medicine2500-07642542-0941КемГМУ10.23946/2500-0764-2020-5-4-104-115fcmedicine-344Research ArticleОБЗОРНЫЕ СТАТЬИREVIEW ARTICLESХимические механизмы действия холодной плазмы на клеткиChemical mechanisms of non-thermal plasma action on cellshttps://orcid.org/0000-0002-7902-7876ОловянниковаР. Я.OlovyannikovaR. Ya.

Оловянникова Раиса Яковлевна, кандидат биологических наук, доцент, доцент кафедры биологической химии с курсом медицинской, фармацевтической и токсикологической химии

660022, г. Красноярск, Партизана Железняка ул., 1

Raisa Ya. Olovyannikova, PhD, Associate Professor, Department of Biological, Medical, Pharmaceutical and Toxicological Chemistry

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0002-2899-8103МакаренкоТ. А.MakarenkoТ. A.

Макаренко Татьяна Александровна, доктор медицинских наук, заведующий кафедрой оперативной гинекологии института последипломного образования

660022, г. Красноярск, Партизана Железняка ул., 1

Tatiana A. Makarenko, MD, DSc, Head of the Department of Operative Gynecology, Institute of Postgraduate Education

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0002-4017-1125ЛычковскаяЕ. В.LychkovskayaE. V.

Лычковская Елена Викторовна, старший преподаватель кафедры биохимии с курсами медицинской, фармацевтической и токсикологической химии

660022, г. Красноярск, Партизана Железняка ул., 1

Elena V. Lychkovskaya, Senior Lecturer, Department of Biological, Medical, Pharmaceutical and Toxicological Chemistry

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0002-7540-4144ГудковаЕ. С.GudkovaE. S.

Гудкова Елизавета Сергеевна, лаборант лаборатории медицинской кибернетики и управления в здравоохранении

660022, г. Красноярск, Партизана Железняка ул., 1

Elizaveta S. Gudkova, Research Assistant, Laboratory of Medical Cybernetics Healthcare Management

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0002-5656-4636МурадянГ. А.MuradyanG. A.

Мурадян Гоар Аматуновна, лаборант лаборатории медицинской кибернетики и управления в здравоохранении

660022, г. Красноярск, Партизана Железняка ул., 1

Goar A. Muradyan, Research Assistant, Laboratory of Medical Cybernetics and Healthcare Management

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0002-7757-6628МедведеваН. Н.MedvedevaN. N.

Медведева Надежда Николаевна, доктор медицинских наук, профессор, заведующая кафедрой анатомии человека

660022, г. Красноярск, Партизана Железняка ул., 1

Nadezhda N. Medvedeva, MD, DSc, Professor, Head of the Department of Human anatomy

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0001-6905-980XЧекишеваТ. Н.ChekishevaТ. N.

Чекишева Татьяна Николаевна, ассистент кафедры гистологии, цитологии, эмбриологии

660022, г. Красноярск, Партизана Железняка ул., 1

 

Tatiana N. Chekisheva, Assistant Professor, Department of Histology, Cytology and Embryology

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0002-9196-589XБердниковС. И.BerdnikovS. I.

Бердников Сергей Иванович, врач-эндоскопист

660037, г. Красноярск, Коломенская ул., 26

Sergey I. Berdnikov, MD, Endoscopist, Federal Siberian Research Clinical Centre, 

26, Kolomenskaya Street, Krasnoyarsk, 660037

https://orcid.org/0000-0003-2386-5798СемичевЕ. В.SemichevE. V.

Семичев Евгений Васильевич, доктор медицинских наук, врачэндоскопист, руководитель научного отдела

60037, г. Красноярск, Коломенская ул., 26

Evgeniy V. Semichev, MD, DSc, endoscopist, Head of the Research Department, The Federal State-Financed Institution Federal Siberian Research Clinical Centre

26, Kolomenskaya Street, Krasnoyarsk, 660037

https://orcid.org/0000-0002-0033-3804МалиновскаяН. А.MalinovskayaN. A.

Малиновская Наталия Александровна, доктор медицинских наук, профессор кафедры биологической химии с курсом медицинской, фармацевтической и токсикологической химии, старший научный сотрудник научно-исследовательского института молекулярной медицины и патобиохимии

660022, г. Красноярск, Партизана Железняка ул., 1

 

Natalia A. Malinovskaya, MD, DSc, Professor of the Department of Biological, Medical, Pharmaceutical and Toxicological Chemistry; Senior Researcher, Research Institute of Molecular Medicine and Pathological Biochemistry

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0003-4012-6348СалминаА. Б.SalminaA. B.

Салмина Алла Борисовна, доктор медицинских наук, заведующая кафедрой биологической химии с курсом медицинской, фармацевтической и токсикологической химии, руководитель научно-исследовательского института молекулярной медицины и патобиохимии

660022, г. Красноярск, Партизана Железняка ул., 1

 

Alla B. Salmina, MD, DSc, Head of the Department of Biological, Medical, Pharmaceutical and Toxicological Chemistry, Head of the Research Institute of Molecular Medicine and Pathological Biochemistry

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

https://orcid.org/0000-0003-4441-9025СалминВ. В.SalminV. V.

Салмин Владимир Валерьевич, доктор физико-математических наук, заведующий кафедрой медицинской и биологической физики

660022, г. Красноярск, Партизана Железняка ул., 1

 

Vladimir V. Salmin, DSc, Head of the Department of Medical and Biological Physics

1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022

ФГБОУ ВО «Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого» Министерства здравоохранения Российской ФедерацииРоссияProf. V.F. Voino-Yasenetsky Krasnoyarsk State Medical UniversityRussian FederationФГБОУ ВО «Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого» Министерства здравоохранения Российской ФедерацииРоссияProf. V.F. Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical UniversityRussian FederationФГБУ «Федеральный Сибирский научно-клинический центр Федерального медико-биологического агентства»РоссияFederal Medical Biological AgencyRussian FederationФГБУ «Федеральный Сибирский научно-клинический центр Федерального медикобиологического агентства»РоссияFederal Medical Biological AgencyRussian FederationФГБОУ ВО «Красноярский государственный медицинский университет имени профессора В.Ф. ВойноЯсенецкого» Министерства здравоохранения Российской ФедерацииРоссияProf. V.F. Voino-Yasenetsky Krasnoyarsk State Medical UniversityRussian Federation20202412202054104116Copyright © Оловянникова Р.Я., Макаренко Т.А., Лычковская Е.В., Гудкова Е.С., Мурадян Г.А., Медведева Н.Н., Чекишева Т.Н., Бердников С.И., Семичев Е.В., Малиновская Н.А., Салмина А.Б., Салмин В.В., 20202020Оловянникова Р.Я., Макаренко Т.А., Лычковская Е.В., Гудкова Е.С., Мурадян Г.А., Медведева Н.Н., Чекишева Т.Н., Бердников С.И., Семичев Е.В., Малиновская Н.А., Салмина А.Б., Салмин В.В.Olovyannikova R.Y., Makarenko Т.A., Lychkovskaya E.V., Gudkova E.S., Muradyan G.A., Medvedeva N.N., Chekisheva Т.N., Berdnikov S.I., Semichev E.V., Malinovskaya N.A., Salmina A.B., Salmin V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fcm.kemsmu.ru/jour/article/view/344

Холодная плазма (ХП) в воздухе над поверхностью суспензий клеток или биологических тканей рассматривается как генератор активных форм кислорода и азота, ионов, сольватированных/акватированных электронов. В обзоре литературы проанализированы современные представления о реализации эффектов ХП в живых системах (с акцентом на роль свободных радикалов и других частиц, генерируемых ХП, в химической модификации биомакромолекул и регуляции сигнальной трансдукции в клетках). Обобщены данные о влиянии продуктов ХП на внутриклеточный окислительно-восстановительный баланс, митохондриальный биогенез, а также клеточные мембраны и органеллы. Обсуждаются ключевые механизмы транспорта продуктов ХП через биологические мембраны с участием механизмов активного транспорта и диффузии. Предполагается, что экспрессия различных транспортных систем будет существенным образом отличаться в клетках на разных стадиях развития, с разным пролиферативным потенциалом, а также в условиях патологии, что определяет важность экспериментальных исследований на разнообразных модельных клеточных системах для оценки того, насколько данная популяция клеток чувствительна к эффектам продуктов, генерируемых ХП. Обсуждаются варианты распространения эффектов ХП вглубь ткани, если генерируемые ХП короткоживущие частицы действуют на клетки ткани с ее поверхности. Приведены данные о том, что воздействие ХП на ткань может быть осуществлено не только непосредственно разрядом, но и растворами, обработанными ХП (фосфатный буфер, раствор Рингера, питательная среда), состав и рН которых после обработки ХП будут существенно различаться, что определяет новые возможности применения самой ХП и растворов, обработанных ХП, в медицине. Изучение молекулярных механизмов действия ХП на биологические системы включает анализ событий, связанных с генерацией и аккумуляцией активных форм кислорода, нейтральных соединений, сольватированных электронов, идентификацию новых клеточных мишеней их действия, что обеспечит создание эффективных и безопасных протоколов применения ХП в биологии и медицине.

Non-thermal plasma (NTP) in the air around the cell layer or biological tissues is considered as a generator of reactive oxygen and nitrogen species, ions, and solvated/aquated electrons. This review covers current understanding on the effects of NTP in living systems, with the focus on the role of free radicals and other NTP-generated particles in the chemical modification of biomacromolecules and regulation of signal transduction. We summarise recent data on the impact of NTP-originated products on intracellular redox balance, mitochondrial biogenesis, cell membranes and organelles. In addition, we discuss the transport of NTP products across the biological membranes. Since the expression of numerous transporter systems differs at various stages of development, distinct cell lines, and in pathological conditions, experiments on NTP effects should be designed in various models for the assessment of cell- and tissue-specific response. Notably, NTP effects are observed throughout the whole tissue even when particles are generated at the surface. Special attention is paid to the NTP-treated solutions (phosphate buffered saline, Ringer’s solution, cell culture medium) as their composition and pH can be significantly altered. However, these data also suggest novel opportunities for the application of NTP and NTP-treated solutions in biomedicine. Studies on the mechanisms of NTP action on biological systems should contain analysis of events coupled to generation and accumulation of reactive oxygen and nitrogen species, neutral compounds, solvated electrons, and detection of new cellular targets of their action. This would allow developing of efficient and safe protocols for NTP applications in biology and medicine.

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The authors declare that there are no conflicts of interest present.