fcmedicineФундаментальная и клиническая медицинаFundamental and Clinical Medicine2500-07642542-0941КемГМУ10.23946/2500-0764-2022-7-3-17-22fcmedicine-568Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL RESEARCHПатогенетическое значение трансформирующего фактора роста β1 у больных колоректальным ракомPathogenetic significance of transforming growth factor β1 in patients with colorectal cancerhttps://orcid.org/0000-0002-8472-107XЧетверяковА. В.ChetveryakovA. V.

Четверяков Андрей Валерьевич, аспирант кафедры госпитальной хирургии; врач-хирург торакоабдоминального хирургического отделения ГУЗ «Забайкальский краевойонкологический диспансер»

 672000, Россия, г. Чита, ул. Горького, д. 39а 

Dr. Andrey V. Chetveryakov, MD, PhD Student, Department of Hospital Surgery; Surgeon, Surgery Unit, Chita Regional Cancer Centre

39a, Gor’kogo Street, Chita, 672000, Russian Federation

104, Leningradskaya Street, Chita, 672027, Russian Federation

yasnogorsk94@gmail.comhttps://orcid.org/0000-0002-2166-5154ЦепелевВ. Л.TsepelevV. L.

Цепелев Виктор Львович, доктор медицинских наук, профессор, заведующий кафедрой госпитальной хирургии  

 672000, Россия, г. Чита, ул. Горького, д. 39а 

Prof. Victor L. Tsepelev, MD, DSc, Professor, Head of the Department ofHospital Surgery

39a, Gor’kogo Street, Chita, 672000, Russian Federation

ФГБОУ ВО «Читинская государственная медицинская академия» Министерства здравоохранения Российской ФедерацииРоссияChita State Medical AcademyRussian Federation202230092022731722Copyright © Четверяков А.В., Цепелев В.Л., 20222022Четверяков А.В., Цепелев В.Л.Chetveryakov A.V., Tsepelev V.L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fcm.kemsmu.ru/jour/article/view/568

Цель. Оценка уровня TGF-β1 в сыворотке крови, ткани опухоли и лимфатических узлов у пациентов с новообразованиями толстого кишечника.Материалы и методы. Исследование выполнено на базе ГУЗ «Забайкальский краевой онкологический диспансер», г. Чита, включало 44 пациента с колоректальным раком, а также 25 больных с доброкачественными новообразованиями толстой кишки, проходивших лечение в период с 2019 по 2020 гг. Контрольная группа включала 25 пациентов, проходивших лечение (пластика колостомы) в ГУЗ «Краевая клиническая больница» г. Чита. Концентрацию TGF-β1 определяли в сыворотке крови, в супернатанте гомогената ткани опухоли и лимфатических узлов с помощью метода проточной цитофлуометрии на анализаторе CytoFlex LX (Beckman Coulter, США), используя набор для мультиплексного анализа LEGENDplex™ HU (Immune Checkpoint, США).Результаты. Установлено, что уровень TGF-β1 в сыворотке крови у пациентов с раком толстой кишки был меньше в 1,38 [1,38; 1,50] раза по сравнению с группой контроля. Концентрация TGF-β1 в сыворотке крови у больных с раком толстой кишки ниже по отношению к группе пациентов с доброкачественными новообразованиями толстого кишечника в 1,58 [1,23; 2,01] раза. Показано, что уровень TGF-β1 в опухолевой ткани у пациентов с раком толстой кишки больше в 5,91 [3,86; 7,81] раза, чем в группе контроля. При сравнении концентрации TGF-β1 у пациентов с раком толстой кишки и больными с доброкачественными новообразованиями толстого кишечника, статистических различий выявлено не было.Заключение. Полученные данные демонстрируют увеличение уровня TGF-β1 в ткани опухоли у пациентов с колоректальным раком. В то же время концентрация TGF-β1 в сыворотке крови у больных с раком толстой кишки ниже чем в контрольной группе.

Aim. We aimed to evaluate levels of transforming growth factor β1 (TGF-β1) in the serum, lymph nodes, and primary tumour in patients with colorectal cancer.Materials and Methods. Here we enrolled 44 patients with colorectal cancer and 25 patients with benign tumours of the colon admitted to Chita Regional Cancer Centre in 2019-2020. The control group included 25 patients with colon injury. The concentration of TGF-β1 in the serum, lymph nodes, and tumour homogenate was measured by flow cytometry (CytoFlex LX analyzer and LEGENDplex HU multiplex analysis kit).Results. Serum level of TGF-β1 in patients with colorectal cancer was 1.58-fold lower than in those with benign colon tumours and 1.38-fold lower than in the control group. In contrast, TGF-β1 level in tumor tissue was 5.91 (3.86; 7.81) fold higher than in the injured colonic tissue from the control group, although there were no statistically significant differences between the cancerous tissue and benign neoplasms.Conclusion. TGF-β1 is increased in tumour tissue but reduced in the serum of patients with colorectal cancer.

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The authors declare that there are no conflicts of interest present.