fcmedicineФундаментальная и клиническая медицинаFundamental and Clinical Medicine2500-07642542-0941КемГМУ10.23946/2500-0764-2024-9-2-8-19fcmedicine-859Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL RESEARCHЭкспрессия Beclin-1 и Caspase 3 в посттравматическом периоде экспериментального ушиба сердца у крыс с различной стрессоустойчивостьюExpression of Beclin-1 and Caspase-3 After the Myocardial Contusion in Rats With High and Low Stress Resistancehttps://orcid.org/0000-0003-4606-3173КлючниковаЕ. И.KlyuchnikovaE. I.

Ключникова Евгения Игоревна, аспирант кафедры патофизиологии

644099, г. Омск, ул. Ленина, д. 12 

Dr. Evgenia I. Klyuchnikova, MD, PhD Student, Department of Pathophysiology

12, Lenina Street, Omsk, 644099 

kei_omsk@mail.ruhttps://orcid.org/0000-0001-6110-3933КорпачеваО. В.KorpachevaO. V.

Корпачева Ольга Валентиновна, доктор медицинских наук, доцент, заведующая кафедрой патофизиологии

644099, г. Омск, ул. Ленина, д. 12 

Prof. Olga V. Korpacheva, MD, DSc, Head of the Department of Pathophysiology 

12, Lenina Street, Omsk, 644099 

https://orcid.org/0000-0001-7200-7082МозговойС. И.MozgovoiS. I.

Мозговой Сергей Игоревич, доктор медицинских наук, доцент, профессор кафедры патологической анатомии

644099, г. Омск, ул. Ленина, д. 12 

Prof. Sergei I. Mozgovoi, MD, DSc, Professor, Department of Pathological Anatomy

12, Lenina Street, Omsk, 644099 

https://orcid.org/0000-0002-6775-323XЗолотовА. Н.ZolotovA. N.

Золотов Александр Николаевич, кандидат медицинских наук, доцент кафедры патофизиологии

644099, г. Омск, ул. Ленина, д. 12 

Dr. .Alexander N. Zolotov, MD, PhD, Associate Professor, Department of Pathophysiology

12, Lenina Street, Omsk, 644099 

https://orcid.org/0000-0001-8607-7831КононовА. В.KononovA. V.

Кононов Алексей Владимирович, доктор медицинских наук, профессор, заслуженный деятель науки РФ, заведующий кафедрой патологической анатомии

644099, г. Омск, ул. Ленина, д. 12 

Prof. Alexei V. Kononov, MD, DSc, Professor, Honored Scientist of the Russian Federation, Head of the Department of Pathological Anatomy

12, Lenina Street, Omsk, 644099 

ФГБОУ ВО «Омский государственный медицинский университет» Министерства здравоохранения Российской ФедерацииРоссияOmsk State Medical UniversityRussian Federation20242006202492819Copyright © Ключникова Е.И., Корпачева О.В., Мозговой С.И., Золотов А.Н., Кононов А.В., 20242024Ключникова Е.И., Корпачева О.В., Мозговой С.И., Золотов А.Н., Кононов А.В.Klyuchnikova E.I., Korpacheva O.V., Mozgovoi S.I., Zolotov A.N., Kononov A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fcm.kemsmu.ru/jour/article/view/859Цель

Цель. Оценка активности аутофагии и апоптоза в миокарде крыс с различной стрессоустойчивостью после моделирования ушиба сердца.

Материалы и методы

Материалы и методы. Исследование проводили на 106 белых беспородных крысах-самцах массой 250–300 г. Крыс ранжировали по стрессоустойчивости, среднеустойчивых особей (n = 42) исключили из эксперимента. Из оставшихся животных сформировали контрольную (n = 16) и опытную (n = 48) группы, в контрольную вошли подгруппы с высокой и низкой стрессоустойчивостью, в опытную включили шесть подгрупп в соответствии с тремя исследовательскими точками посттравматического периода: с высокой и низкой стрессоустойчивостью и длительностью эксперимента 6, 12 и 24 часа. Таким образом сформировали восемь подгрупп по 8 животных в каждой. В опытной группе моделировали ушиб сердца. Через 6, 12 и 24 часа соответственно сердца крыс извлекали, далее из зон, подверженных наибольшему травматическому воздействию (межжелудочковой перегородки, передних стенок левого и правого желудочков), иссекали фрагменты миокарда 5х5 мм, изготавливали гистологические срезы и проводили иммуногистохимическое исследование с антителами к маркерам Beclin-1 и Caspase 3. Полученные образцы исследовали под микроскопом.

Результаты

Результаты. В миокарде крыс после ушиба сердца была выявлена экспрессия маркеров Beclin-1 и Caspase 3. У крыс с высокой стрессоустойчивостью отмечалось нарастание индекса экспрессии Beclin-1 в динамике посттравматического периода, а у животных с низкой стрессоустойчивостью наблюдалось постепенное снижение. У высокоустойчивых крыс экспрессия Caspase 3 регистрировалась только через 24 часа после травмы, а у низкоустойчивых животных наблюдалось нарастание экспрессии в динамике посттравматического периода.

Заключение

Заключение. Экспрессия Beclin-1 в миокарде крыс опытной группы свидетельствует о запуске аутофагии, а экспрессия Caspase 3 – об активации апоптоза. Выраженность данных реакций неодинакова у животных с разной стрессоустойчивостью.

Aim

Aim. To evaluate the activity of autophagy and apoptosis in the myocardium of rats with differentstress resistance after the modeling of myocardial contusion.

Materials and Methods

Materials and Methods. The study was performed on 106 white male rats weighing 250-300 g. The rats were ranked according to stress tolerance, and medium-resistant individuals (n = 42) were excluded from the experiment. Control (n = 16) and experimental (n = 48) groups were formed from the remaining animals; the control group included subgroups with high and low stress resistance, and the experimental group included 6 subgroups (rats with high and low stress resistance; 6, 12, and 24-hour time points). Each of the subgroups included 8 animals. Myocardial contusion was modeled in the experimental group. At 6-, 12- and 24-hour time point, rat hearts have been excised and 5×5 mm myocardial fragments were dissected from the areas with the most prominent traumatic effects (interventricular septum, anterior walls of the left and right ventricles). Tissues were then sectioned and stained with antibodies to Beclin-1 and caspase-3.

Results

Results. We have documented a significant expression of Beclin-1 and Caspase 3 expression in rat hearts after myocardial contusion. From 6 to 24 hours upon the myocardial contusion, Beclin-1 expression has been increased in rats with high stress resistance but was reduced in rats with low stress resistance. Expression of caspase-3 expression was registered exclusively at 24-hour time point in rats with high stress resistant but increased along the time points in rats with low stress resistance.

Conclusion

Conclusion. Expression of Beclin-1 and caspase-3 in rat myocardium indicated autophagy and apoptosis upon the myocardial contusion. Temporal patterns of Beclin-1 and caspase-3 expression were opposite in rats with high and low stress resistance.

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The authors declare that there are no conflicts of interest present.