fcmedicineФундаментальная и клиническая медицинаFundamental and Clinical Medicine2500-07642542-0941КемГМУ10.23946/2500-0764-2024-9-3-86-97fcmedicine-904Research ArticleОБЗОРНЫЕ СТАТЬИREVIEW ARTICLESРоль факторов иммунного ответа в развитии рака лёгкого у пациентов в зависимости от статуса курения (систематический обзор)Immune response, smoking, and lung cancer: a systematic reviewhttps://orcid.org/0000-0001-5566-5323БуслаевВ. Ю.BuslaevV. Yu.

Буслаев Владислав Юрьевич, ведущий инженер лаборатории цитогенетики 

650056, Россия, г. Кемерово, Ленинградский пр-т., д. 10

Mr. Vladislav Yu. Buslaev, Leading Engineer, Laboratory of Cytogenetics

10, Leningradskiy Prospekt, Kemerovo, 650056

Vladislasbus2358@yandex.ruhttps://orcid.org/0000-0003-3871-5450МининА. В.MininA. V.

Минин Артем Вячеславович, инженер лаборатории цитогенетики; аспирант кафедры генетики и фундаментальной медицины

650056, г. Кемерово, Ленинградский пр., д. 10; 650056, г. Кемерово, ул. Красная, д. 6

Mr. Artem V. Minin, Engineer, Laboratory of Cytogenetics; ; PhD Student, Department of Genetics and Basic Medicine

10, Leningradskiy Prospekt, Kemerovo, 650056; 6, Krasnaya Street, Kemerovo, 650056

https://orcid.org/0009-0003-9165-2716КатанаховаМ. В.KatanakhovaM. V.

Катанахова Маргарита Владиславовна, инженер лаборатории цитогенетики; аспирант кафедры генетики и фундаментальной медицины

650056, г. Кемерово, Ленинградский пр., д. 10; 650056, г. Кемерово, ул. Красная, д. 6

Mrs. Margarita V. Katanakhova, Engineer, Laboratory of Cytogenetics; PhD Student, Department of Genetics and Basic Medicine

10, Leningradskiy Prospekt, Kemerovo, 650056; 6, Krasnaya Street, Kemerovo, 650056

https://orcid.org/0000-0002-8785-7896ШабалдинА. В.ShabaldinA. V.

Шабалдин Андрей Владимирович, доктор медицинских наук, профессор кафедры поликлинической педиатрии, пропедевтики детских болезней и последипломной подготовки; профессор кафедры генетики и фундаментальной медицины

65056, г. Кемерово, ул. Ворошилова, д. 22а; 650056, г. Кемерово, ул. Красная, д. 6

Prof. Andrey V. Shabaldin, MD, PhD, Professor, Department of Polyclinic Pediatrics, Introduction to Pediatrics and Postgraduate Training; Professor, Department of Genetics and Basic Medicine

22a, Voroshilova Street, Kemerovo, 650056; 6, Krasnaya Street, Kemerovo, 650056

Институт экологии человека Федерального исследовательского центра угля и углехимии СО РАНРоссияInstitute of Human Ecology, Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of SciencesRussian FederationИнститут экологии человека Федерального исследовательского центра угля и углехимии СО РАН; ФГБОУ ВО «Кемеровский государственный университет»РоссияInstitute of Human Ecology, Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences; Kemerovo State UniversityRussian FederationФГБОУ ВО «Кемеровский государственный университет»; ФГБОУ ВО «Кемеровский государственный медицинский университет» Министерства здравоохранения Российской ФедерацииРоссияKemerovo State University; Kemerovo State Medical UniversityRussian Federation202427092024938697Copyright © Буслаев В.Ю., Минин А.В., Катанахова М.В., Шабалдин А.В., 20242024Буслаев В.Ю., Минин А.В., Катанахова М.В., Шабалдин А.В.Buslaev V.Y., Minin A.V., Katanakhova M.V., Shabaldin A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://fcm.kemsmu.ru/jour/article/view/904

Выполнен анализ роли факторов иммунного ответа в развитии рака лёгкого у пациентов в зависимости от статуса курения. В результате анализа данных литературы были обобщены сведения о роли врождённого и приобретенного иммунитета в развитии рака лёгкого, охарактеризована их роль на разных стадиях развития болезни. Были описаны основные свойства компонентов иммунного ответа в зависимости от статуса курения у пациентов. Активность Т-клеточного звена и экспрессия провоспалительных цитокинов были повышены у курильщиков. В результате курение было представлено как значимый фактор риска, который, инициируя воспалительные реакции, может влиять на канцерогенез в легких. Проведение последующих экспериментальных исследований в данном направлении позволит сформулировать новые диагностические подходы. Целесообразным является изучение характеристик генов факторов иммунитета в качестве биологических маркеров.

Here we review the role of innate and adaptive immunity at ascending lung cancer stages in smoking and non-smoking patients. T cell activity and the expression of pro-inflammatory cytokines were found to be elevated in smokers. As a consequence, smoking has been identified as a significant risk factor promoting lung carcinogenesis. Further experimental research in this area will allow the development of novel diagnostic approaches, including circulating biomarkers and personalised medicine.

обзоркурениефакторы иммунитетарак лёгкогоreviewsmokingimmunity factorslung cancerИсследование выполнено в рамках соглашения о создании молодёжной лаборатории (лаборатория онкогеномики) ФИЦ УУХ СО РАН в связи с постановлением Правительства Кемеровской области-Кузбасса от 19.09.2022 №623.This study was funded by the Ministry of Science and Higher Education of Kemerovo Region – Kuzbass (decree #623 from 19.09.2022).ReferencesRelli V., Trerotola M., Guerra E., Alberti S. Abandoning the Notion of Non-Small Cell Lung Cancer. Trends Mol. Med. 2019;25(7):585-594. https://doi.org/10.1016/j.molmed.2019.04.012Relli V, Trerotola M, Guerra E, Alberti S. Abandoning the Notion of Non-Small Cell Lung Cancer. Trends Mol Med. 2019;25(7):585-594. https://doi.org/10.1016/j.molmed.2019.04.012Hosseini B., Olsson A., Bouaoun L., Hall A., Hadji M., Rashidian H., Naghibzadeh-Tahami A., Marzban M., Najafi F., Haghboost A.A., Bofetta P., Kamangar F., Pukkala E., Etemadi A., Weiderpass E., Schuz J., Zendehdel K. Lung cancer risk in relation to jobs held in a nationwide case–control study in Iran. Occup. Environ. Med. 2022;79(12):831-838. https://doi.org/10.1136/oemed-2022-108463Hosseini B, Olsson A, Bouaoun L, Hall A, Hadji M, Rashidian H, Naghibzadeh-Tahami A, Marzban M, Najafi F, Haghboost AA, Bofetta P, Kamangar F, Pukkala E, Etemadi A, Weiderpass E, Schuz J, Zendehdel K. Lung cancer risk in relation to jobs held in a nationwide case–control study in Iran. Occup Environ Med. 2022;79(12):831-838. https://doi.org/10.1136/oemed-2022-108463Singh A., Kamal R., Ahamed I., Wagh M., Bihari V., Sathian B., Kesavachandran C.N. PAH exposure-associated lung cancer: an updated meta-analysis. Occup. Med. 2018;68(4):255-261. https://doi.org/10.1093/occmed/kqy049Singh A, Kamal R, Ahamed I, Wagh M, Bihari V, Sathian B, Kesavachandran CN. PAH exposure-associated lung cancer: an updated meta-analysis. Occup Med. 2018;68(4):255-261. https://doi.org/10.1093/occmed/kqy049Ларионов А.В., Волобаев В.П., Сердюкова Е.С. Исследование анеугенных эффектов с помощью панцентромерных зондов в условиях резидентного воздействия радона. Известия Самарского научного центра Российской академии наук. 2018;20(5-4):614-620.Larionov A, Volobaev V, Serdyukova E. Application of the impedance method for comparative research of the chronic chemical stress impact on the development of the gastrointestinal tract pathology of male and female white outbred rats. Izvestija Samarskogo nauchnogo centra Rossijskoj akademii nauk. 2018;20(5-4):614-620. (In Russian).Бабанов С.А., Будаш Д.С., Байкова А.Г., Рыжова Н.С. Профессиональные злокачественные новообразования лёгких и других локализаций и потенциально опасные канцерогенные продукты. CONSILIUM MEDICUM. 2017;19(11):39-46. https://doi.org/10.26442/2075-1753_19.11.39-46Babanov SA, Budash DS, Baykova AG, Ryzhova NS. Occupational malignant tumors of the lungs and other organs and potentially dangerous industrial carcinogens. CONSILIUM MEDICUM. 2017;19(11):39-46. (In Russian). https://doi.org/10.26442/2075-1753_19.11.39-46Subramanian J., Govindan R. Molecular profile of lung cancer in never smokers. EJC Suppl. 2013:11(2):248-253. https://doi.org/10.1016/j.ejcsup.2013.07.004Subramanian J, Govindan R. Molecular profile of lung cancer in never smokers. EJC Suppl. 2013:11(2):248-253. https://doi.org/10.1016/j.ejcsup. 2013. 07.004Dong Y., Ren W., Qi J., Jin B.O., Li Y., Tao H., Xu R., Li Y., Zhang Q., Han B. EGFR, ALK, RET, KRAS and BRAF alterations in never-smokers with non-small cell lung cancer. Oncol. Lett. 2016;11(4):2371-2378. https://doi.org/10.3892/ol.2016.4235Dong Y, Ren W, Qi J, Jin BO, Li Y, Tao H, Xu R, Li Y, Zhang Q, Han B. EGFR, ALK, RET, KRAS and BRAF alterations in never-smokers with non-small cell lung cancer. Oncol Lett. 2016;11(4):2371-2378. https://doi.org/10.3892/ol.2016.4235Banat G.A., Tretyn A., Pullamsetti S.S., Wilhelm J., Weigert A., Olesch C., Ebel K., Stiewe T., Grimminger F., Seeger W., Fink L., Savai R. Immune and Inflammatory Cell Composition of Human Lung Cancer Stroma. PLoS ONE. 2015;10(9):e0139073. https://doi.org/10.1371/journal.pone.0139073Banat GA, Tretyn A, Pullamsetti SS, Wilhelm J, Weigert A, Olesch C, Ebel K, Stiewe T, Grimminger F, Seeger W, Fink L, Savai R. Immune and Inflammatory Cell Composition of Human Lung Cancer Stroma. PLoS ONE. 2015;10(9):e0139073. https://doi.org/10.1371/journal.pone.0139073Stankovic B., Bjørhovde H.A.K., Skarshaug R., Aamodt H., Frafjord A., Muller E, Hammastrom C., Beraki K., Bakkewold E.S., Woldbak P.R., Helland A., Brustugun O.T., Oynebraten I., Corthay A. Immune Cell Composition in Human Non-small Cell Lung Cancer. Front. Immunol. 2019;9:3101. https://doi.org/10.3389/fimmu.2018.03101Stankovic B, Bjørhovde HAK, Skarshaug R, Aamodt H, Frafjord A, Muller E, Hammastrom C, Beraki K, Bakkewold ES, Woldbak PR, Helland A, Brustugun OT, Oynebraten I, Corthay A. Immune Cell Composition in Human Non-small Cell Lung Cancer. Front Immunol. 2019;9:3101. https://doi.org/10.3389/fimmu.2018.03101Saab S., Zalzale H., Rahal Z., Khalifeh Y., Sinjab A., Kadara H. Insights Into Lung Cancer Immune-Based Biology, Prevention, and Treatment. Front. Immunol. 2020;11:159. https://doi.org/10.3389/fimmu.2020.00159Saab S, Zalzale H, Rahal Z, Khalifeh Y, Sinjab A, Kadara H. Insights Into Lung Cancer Immune-Based Biology, Prevention, and Treatment. Front Immunol. 2020;11:159. https://doi.org/10.3389/fimmu.2020.00159Whitsett J.A., Wert S.E., Weaver T.E. Diseases of Pulmonary Surfactant Homeostasis. Annu. Rev. Pathol. 2015;10(1):371-393. https://doi.org/10.1146/annurev-pathol-012513-104644Whitsett JA, Wert SE, Weaver TE. Diseases of Pulmonary Surfactant Homeostasis. Annu Rev Pathol 2015;10(1):371-393. https://doi.org/10.1146/annurev-pathol-012513-104644Tengroth L., Millrud C.R., Kvarnhammar A.M., Kumlien Georén S., Latif L., Cardell L.O. Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells. PLoS ONE. 2014;9(6):e98239. https://doi.org/10.1371/journal.pone.0098239Tengroth L, Millrud CR, Kvarnhammar AM, Kumlien Georén S, Latif L, Cardell LO. Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells. PLoS ONE. 2014;9(6):e98239. https://doi.org/10.1371/journal.pone.0098239Milette S., Fiset P.O., Walsh L.A., Spicer J.D., Quail D.F. The innate immune architecture of lung tumors and its implication in disease progression. J. Pathol. 2019;247(5):589-605. https://doi.org/10.1002/path.5241Milette S, Fiset PO, Walsh LA, Spicer JD, Quail DF. The innate immune architecture of lung tumors and its implication in disease progression. J Pathol. 2019;247(5):589-605. https://doi.org/10.1002/path.5241Ohue Y., Nishikawa H. Regulatory T (Treg) cells in cancer: Can Treg cells be a new therapeutic target? Cancer Sci. 2019;110(7):2080-2089. https://doi.org/10.1111/cas.14069Ohue Y, Nishikawa H. Regulatory T (Treg) cells in cancer: Can Treg cells be a new therapeutic target? Cancer Sci. 2019;110(7):2080-2089. https://doi.org/10.1111/cas.14069Chen P., Li Z., Liang Y., Wei M., Jiang H., Chen S., Zhao Z. Identification of Hypoxia-Associated Signature in Colon Cancer to Assess Tumor Immune Microenvironment and Predict Prognosis Based on 14 Hypoxia-Associated Genes. Int. J. Gen. Med. 2023;16:2503-2518. https://doi.org/10.2147/IJGM.S407005Chen P, Li Z, Liang Y, Wei M, Jiang H, Chen S, Zhao Z. Identification of Hypoxia-Associated Signature in Colon Cancer to Assess Tumor Immune Microenvironment and Predict Prognosis Based on 14 Hypoxia-Associated Genes. Int J Gen Med. 2023;16:2503-2518. https://doi.org/10.2147/IJGM.S407005Huang C.C., Wang C.H., Fu C.H., Huang C.C., Chang P.H., Chen Y.W., Wu C.C., Wu P.W., Lee T.J. Association between cigarette smoking and interleukin-17A expression in nasal tissues of patients with chronic rhinosinusitis and asthma. Medicine. 2016;95(47):e5432. https://doi.org/10.1097/MD.0000000000005432Huang CC, Wang CH, Fu CH, Huang CC, Chang PH, Chen YW, Wu CC, Wu PW, Lee TJ. Association between cigarette smoking and interleukin-17A expression in nasal tissues of patients with chronic rhinosinusitis and asthma. Medicine. 2016;95(47):e5432. https://doi.org/10.1097/MD.0000000000005432Azargoon A., Kharazmkia A., Kordalivand N., Birjandi M., Mir S. Evaluation of exposure to secondhand smoke and serum level of interleukin 18 in non-smokers. Ann Med Surg (Lond). 2022;73:103328-103242. https://doi.org/10.1016/j.amsu.2021Azargoon A, Kharazmkia A, Kordalivand N, Birjandi M, Mir S. Evaluation of exposure to secondhand smoke and serum level of interleukin 18 in non-smokers. Ann Med Surg (Lond). 2022;73:103328-103242. https://doi.org/10.1016/j.amsu.2021Zhu X., Zhan Y., Gu Y., Huang Q., Wang T., Deng Z., Xie J. Cigarette Smoke Promotes Interleukin-8 Production in Alveolar Macrophages Through the Reactive Oxygen Species/Stromal Interaction Molecule 1/Ca2+ Axis. Front. Physiol. 2021;12:733650-73362. https://doi.org/10.3389/fphys.2021Zhu X, Zhan Y, Gu Y, Huang Q, Wang T, Deng Z, Xie J. Cigarette Smoke Promotes Interleukin-8 Production in Alveolar Macrophages Through the Reactive Oxygen Species/Stromal Interaction Molecule 1/Ca2+ Axis. Front Physiol. 2021;12:733650-73362. https://doi.org/10.3389/fphys.2021Sumanasekera Wasana, Wainge Br. Does Cigarette Smoke Cause Interleukin 1 - Beta (Il-1ß) Production in Cardiac Stem Cells? J. Cell Biol. Cell Metab. 2016;3(1):1-6. https://doi.org/10.24966/CBCM-1943/100012Sumanasekera Wasana, Wainge Br. Does Cigarette Smoke Cause Interleukin 1 - Beta (Il-1ß) Production in Cardiac Stem Cells? J. Cell Biol. Cell Metab. 2016;3(1):1-6. https://doi.org/10.24966/CBCM-1943/100012Koo J.B., Han J.S. Cigarette smoke extract-induced interleukin-6 expression is regulated by phospholipase D1 in human bronchial epithelial cells. J. Toxicol. Sci. 2016;41(1):77-89. https://doi.org/10.2131/jts.41.77Koo JB, Han JS. Cigarette smoke extract-induced interleukin-6 expression is regulated by phospholipase D1 in human bronchial epithelial cells. J Toxicol Sci. 2016;41(1):77-89. https://doi.org/10.2131/jts.41.77Jamil A., Rashid A., Majeed A. Correlation between Genotoxicity and Interleukin-6 in Smokers: A Rodent Model. J. Coll. Physicians Surg. Pak. 2018;28(11):821-823. https://doi.org/10.29271/jcpsp.2018.11.821Jamil A, Rashid A, Majeed A. Correlation between Genotoxicity and Interleukin-6 in Smokers: A Rodent Model. J Coll Physicians Surg Pak. 2018;28(11):821-823. https://doi.org/10.29271/jcpsp.2018.11.821Kearley J., Silver J.S., Sanden C., Liu Z., Berlin A.A., White N., Mori M., Pham T.-H., Ward C.K., Criner G.J., Marchetti N., Mustelin T., Erjefalt J.S., Kolbeck R., Humbles A.A. Cigarette Smoke Silences Innate Lymphoid Cell Function and Facilitates an Exacerbated Type I Interleukin-33-Dependent Response to Infection. Immunity. 2015;42(3):566- 579. https://doi.org/10.1016/j.immuni.2015.02.011Kearley J, Silver JS, Sanden C, Liu Z, Berlin AA, White N, Mori M, Pham T-H, Ward CK, Criner GJ, Marchetti N, Mustelin T, Erjefalt JS, Kolbeck R, Humbles AA. Cigarette Smoke Silences Innate Lymphoid Cell Function and Facilitates an Exacerbated Type I Interleukin-33- Dependent Response to Infection. Immunity. 2015;42(3):566-579. https://doi.org/10.1016/j.immuni.2015.02.011Shiels M.S., Shu X.O., Chaturvedi A.K., Gao Y.T., Xiang Y.B., Cai Q., Hu W., Shelton G., Ji B.T., Pinto L.A., Kemp T.J., Rothman N., Zheng W., Hildesheim A., Lan Q. A prospective study of immune and inflammation markers and risk of lung cancer among female never smokers in Shanghai. Carcinogenesis. 2017;38(10):1004-1010. https://doi.org/10.1093/carcin/bgx075Shiels MS, Shu XO, Chaturvedi AK, Gao YT, Xiang YB, Cai Q, Hu W, Shelton G, Ji BT, Pinto LA, Kemp TJ, Rothman N, Zheng W, Hildesheim A, Lan Q . A prospective study of immune and inflammation markers and risk of lung cancer among female never smokers in Shanghai. Carcinogenesis. 2017;38(10):1004-1010. https://doi.org/10.1093/carcin/bgx075Ke W., Zhang L., Dai Y. The role of IL-6 in immunotherapy of non-small cell lung cancer (NSCLC) with immune-related adverse events (irAEs). Thorac. Cancer. 2020;11(4):835-839. https://doi.org/10.1111/1759-7714.13341Ke W, Zhang L, Dai Y. The role of IL‐6 in immunotherapy of non-small cell lung cancer (NSCLC) with immune-related adverse events (irAEs). Thorac Cancer. 2020;11(4):835-839. https://doi.org/10.1111/1759-7714.13341Shill M.C., Biswas B., Islam M., Rima S.S., Ferdausi F.A., Chowdhury Q., Reza H.M., Bepari A.K. Screening of Plasma IL-6 and IL-17 in Bangladeshi Lung Cancer Patients. Heliyon. 2023;9(10):e20471. https://doi.org/10.1101/2022.03.27.22272998Shill MC, Biswas B, Islam M, Rima SS, Ferdausi FA, Chowdhury Q, Reza HM, Bepari AK. Screening of Plasma IL-6 and IL-17 in Bangladeshi Lung Cancer Patients. Heliyon. 2023;9(10):e20471. https://doi.org/10.1101/2022.03.27.22272998Ding X., Zhang J., Liu D., Xu W., Lu D.Y., Zhang L.P., Su B. Serum expression level of IL-6 at the diagnosis time contributes to the long-term prognosis of SCLC patients. J. Cancer. 2018;9(5):792-796. https://doi.org/10.7150/jca.22656Ding X, Zhang J, Liu D, Xu W, Lu DY, Zhang LP, Su B. Serum expression level of IL-6 at the diagnosis time contributes to the longterm prognosis of SCLC patients. J Cancer. 2018;9(5):792-796. https://doi.org/10.7150/jca.22656Brenner D.R., Fanidi A., Grankvist K., Muller D.C., Brennan P., Manjer J., Byrnes G., Hodge A., Severi G., Giles G.G., Johansson M., Johansson M. Inflammatory Cytokines and Lung Cancer Risk in 3 Prospective Studies. Am. J. Epidemiol. 2017;185(2):86-95. https://doi.org/10.1093/aje/kww159Brenner DR, Fanidi A, Grankvist K, Muller DC, Brennan P, Manjer J, Byrnes G, Hodge A, Severi G, Giles GG, Johansson M, Johansson M. Inflammatory Cytokines and Lung Cancer Risk in 3 Prospective Studies. Am J Epidemiol. 2017;185(2):86-95. https://doi.org/10.1093/aje/kww159Wang V., Heffer A., Roztocil E., Feldon S.E., Libby R.T., Woeller C.F., Kuriyan A.E. TNF-α and NF-κB signaling play a critical role in cigarette smoke-induced epithelial-mesenchymal transition of retinal pigment epithelial cells in proliferative vitreoretinopathy. PLoS ONE. 2022;17(9):e0271950. https://doi.org/10.1371/journal.pone.0271950Wang V, Heffer A, Roztocil E, Feldon SE, Libby RT, Woeller CF, Kuriyan AE. TNF-α and NF-κB signaling play a critical role in cigarette smoke-induced epithelial-mesenchymal transition of retinal pigment epithelial cells in proliferative vitreoretinopathy. PLoS ONE. 2022;17(9):e0271950. https://doi.org/10.1371/journal.pone.0271950Ramundo V., Palazzo M.L., Aldieri E. TGF-β as Predictive Marker and Pharmacological Target in Lung Cancer Approach. Cancers. 2023;15(8):2295. https://doi.org/10.3390/cancers15082295Ramundo V, Palazzo ML, Aldieri E. TGF-β as Predictive Marker and Pharmacological Target in Lung Cancer Approach. Cancers. 2023;15(8):2295. https://doi.org/10.3390/cancers15082295Dutta R.K., Chinnapaiyan S., Rasmussen L., Raju S.V., Unwalla H.J. A Neutralizing Aptamer to TGFBR2 and miR-145 Antagonism Rescue Cigarette Smoke- and TGF-β-Mediated CFTR Expression. Mol. Ther. 2019;27(2):442-455. https://doi.org/10.1016/j.ymthe.2018.11.017Dutta RK, Chinnapaiyan S, Rasmussen L, Raju SV, Unwalla HJ. A Neutralizing Aptamer to TGFBR2 and miR-145 Antagonism Rescue Cigarette Smoke- and TGF-β-Mediated CFTR Expression. Mol Ther. 2019;27(2):442-455. https://doi.org/10.1016/j.ymthe.2018.11.017Schupp J.C., Binder H., Jäger B., Cillis G., Zissel G., Muller-Quernheim J., Prasse A. Macrophage Activation in Acute Exacerbation of Idiopathic Pulmonary Fibrosis. PLoS ONE. 2015;10(1):e0116775. https://doi.org/10.1371/journal.pone.0116775Schupp JC, Binder H, Jäger B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A. Macrophage Activation in Acute Exacerbation of Idiopathic Pulmonary Fibrosis. PLoS ONE. 2015;10(1):e0116775. https://doi.org/10.1371/journal.pone.0116775Lugg S.T., Scott A., Parekh D., Naidu B., Thickett D.R. Cigarette smoke exposure and alveolar macrophages: mechanisms for lung disease. Thorax. 2022;77(1):94-101. https://doi.org/10.1136/thoraxjnl-2020-216296Lugg ST, Scott A, Parekh D, Naidu B, Thickett DR. Cigarette smoke exposure and alveolar macrophages: mechanisms for lung disease. Thorax. 2022;77(1):94-101. https://doi.org/10.1136/thoraxjnl-2020-216296Wang Y.D., Li Z., Li F.S. Differences in key genes in human alveolar macrophages between phenotypically normal smokers and nonsmokers: diagnostic and prognostic value in lung cancer. Int. J. Clin. Exp. Pathol. 2020;13(11):2788-2805.Wang YD, Li Z, Li FS. Differences in key genes in human alveolar macrophages between phenotypically normal smokers and nonsmokers: diagnostic and prognostic value in lung cancer. Int. J. Clin. Exp. Pathol. 2020;13(11):2788-2805Pirlog R., Chiroi P., Rusu I., Jurj A.M., Budisan L., Pop-Bica C., Braicu C., Crisan D., Sabourin J.C., Berindan-Neagoe I. Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer. Int. J. Mol. Sci. 2022;23(10):5346. https://doi.org/10.3390/ijms23105346Pirlog R, Chiroi P, Rusu I, Jurj AM, Budisan L, Pop-Bica C, Braicu C, Crisan D, Sabourin JC, Berindan-Neagoe I. Cellular and Molecular Profiling of Tumor Microenvironment and Early-Stage Lung Cancer. Int J Mol Sci. 2022;23(10):5346. https://doi.org/10.3390/ijms23105346De Alencar V.T.L., Figueiredo A.B., Corassa M., Gollob K.J., Cordeiro De Lima V.C. Lung cancer in never smokers: Tumor immunology and challenges for immunotherapy. Front. Immunol. 2022;13:984349. https://doi.org/10.3389/fimmu.2022.984349De Alencar VTL, Figueiredo AB, Corassa M, Gollob KJ, Cordeiro De Lima VC. Lung cancer in never smokers: Tumor immunology and challenges for immunotherapy. Front Immunol. 2022;13:984349. https:// doi.org/10.3389/fimmu.2022.984349Sun Y., Yang Q., Shen J., Zhang N., Luo P., Zhang J. The Effect of Smoking on the Immune Microenvironment and Immunogenicity and Its Relationship With the Prognosis of Immune Checkpoint Inhibitors in Nonsmall Cell Lung Cancer. Front. Cell Dev. Biol. 2021;9:745859. https://doi.org/10.3389/fcell.2021.745859Sun Y, Yang Q, Shen J, Zhang N, Luo P, Zhang J. The Effect of Smoking on the Immune Microenvironment and Immunogenicity and Its Relationship With the Prognosis of Immune Checkpoint Inhibitors in Non-small Cell Lung Cancer. Front Cell Dev Biol. 2021;9:745859. https://doi.org/10.3389/fcell.2021.745859Глушков А.Н., Поленок Е.Г., Гордеева Л.А., Мун С.А., Костянко М.В., Антонов А.В., Вержбицкая Н.Е., Воронина Е.Н., Колпинский Г.И. Иммунологический дисбаланс, генетический полиморфизм ферментов биотрансформации и рецепторы стероидных гормонов в опухоли у больных раком молочной железы. Медицинская иммунология. 2022;24(4):765-778. https://doi.org/10.15789/1563-0625-IIG-2493Glushkov AN, Polenok EG, Gordeeva LA, Mun SA, Kostyanko MV, Antonov AV, Verzhbitskaya NE, Voronina EN, Kolpinskiy GI. Immunological imbalance, gene polymorphism of biotransformation enzymes, and steroid hormone receptors in tumors in breast cancer patients. Medical Immunology (Russia)/Meditsinskaya Immunologiya. 2022;24(4):765-778. (In Russian). https://doi.org/10.15789/1563-0625-IIG-2493Глушков А.Н., Поленок Е.Г., Мун С.А., Гордеева Л.А., Костянко М.В., Антонов А.В., Вержбицкая Н.Е., Вафин И.А. Индивидуальный иммунологический фенотип и риск рака молочной железы у женщин в постменопаузе. Российский иммунологический журнал. 2019;13(1):44-52. https://doi.org/10.31857/S102872210005019-5Glushkov AN, Polenok EG, Mun SA, Gordeeva LA, Kostyanko MV, Antonov AV, Verzhbitskaya NE, Vafin IA. Personal immunological phenotype and breast cancer risk in postmenopausal women. Russian Journal of Immunology. 2019;13(1):44-52. (In Russian). https://doi.org/10.31857/S102872210005019-5Минина В.И., Дружинин В.Г., Ларионов А.В., Баранова Е.Д., Буслаев В.Ю., Мацкова Л.В., Баканова М.Л. Микроматричный анализ транскриптома мононуклеаров периферической крови у пациентов с наличием рака лёгкого. Генетика. 2022;7:814-822. https://doi.org/10.31857/S0016675822070128Minina VI, Druzhinin VG, Larionov AV, Baranova ED, Buslaev VYU., Matskova L.V., Bakanova M.L. Microarray-based transcriptome analysis of peripheral blood mononuclear cells in lung cancer patients. Russian journal of genetics. 2022;7:814-822. (In Russian). https://doi.org/10.31857/S0016675822070128Jeganathan N., Cleland D., Sathananthan M. The association of lung cancer with pulmonary fibrosis. ERJ Open Res. 2022;8(1):00505-02021. https://doi.org/10.1183/23120541.00505-2021Jeganathan N, Cleland D, Sathananthan M. The association of lung cancer with pulmonary fibrosis. ERJ Open Res. 2022;8(1):00505-02021. https://doi.org/10.1183/23120541.00505-2021Cui L., Fang Z., De Souza C.M., Lerbs T., Guan Y., Li I., Charu V., Chen S.Y., Weissman I., Wernig G. Innate immune cell activation causes lung fibrosis in a humanized model of long COVID. Proc. Natl. Acad. Sci. USA. 2023;120(10):e2217199120. https://doi.org/10.1073/pnas.2217199120Cui L, Fang Z, De Souza CM, Lerbs T, Guan Y, Li I, Charu V, Chen SY, Weissman I, Wernig G. Innate immune cell activation causes lung fibrosis in a humanized model of long COVID. Proc Natl Acad Sci USA. 2023;120(10):e2217199120. https://doi.org/10.1073/pnas.2217199120Kärkkäinen M., Kettunen H.P., Nurmi H., Selander T., Purokivi M., Kaarteenaho R. Effect of smoking and comorbidities on survival in idiopathic pulmonary fibrosis. Respir Res. 2017;18(1):160. https://doi.org/10.1186/s12931-017-0642-6Kärkkäinen M, Kettunen HP, Nurmi H, Selander T, Purokivi M, Kaarteenaho R. Effect of smoking and comorbidities on survival in idiopathic pulmonary fibrosis. Respir Res. 2017;18(1):160. https://doi.org/10.1186/s12931-017-0642-6Hao D., Han G., Sinjab A., Gomez-Bolanos L.I., Lazcano R., Serrano A., Hernandez S.D., Dai E., Cao X., Hu J., Dang M., Wang R., Chu Y., Song X., Zhang J., Parra E.R., Wargo J.A., Swisher S.G., Cascone T., Sepesi B., Futreal A.P., Li M., Dubinett S.M., Fujimoto J., Solis Soto L.M., Wistuba I.I., Stevenson C.S., Spira A., Shalapour S., Kadara H., Wang L. The Single-Cell Immunogenomic Landscape of B and Plasma Cells in Early-Stage Lung Adenocarcinoma. Cancer Discov. 2022;12(11):2626- 2645. https://doi.org/10.1158/2159-8290CD-21-1658Hao D, Han G, Sinjab A, Gomez-Bolanos LI, Lazcano R, Serrano A, Hernandez SD, Dai E, Cao X, Hu J, Dang M, Wang R, Chu Y, Song X, Zhang J, Parra ER, Wargo JA, Swisher SG, Cascone T, Sepesi B, Futreal AP, Li M, Dubinett SM, Fujimoto J, Solis Soto LM, Wistuba II, Stevenson CS, Spira A, Shalapour S, Kadara H, Wang L. The SingleCell Immunogenomic Landscape of B and Plasma Cells in Early-Stage Lung Adenocarcinoma. Cancer Discov. 2022;12(11):2626-2645. https://doi.org/10.1158/2159-8290.CD-21-1658Luo W., Zeng Z., Jin Y., Yang L., Fan T., Wang Z., Pan Y., Yang Y., Yao M., Li Y., Xiao X., Wang G., Wang C., Chang S., Che G., Zhang L., Li Y., Peng Y., Li W. Distinct immune microenvironment of lung adenocarcinoma in never-smokers from smokers. Cell. Rep. Med. 2023;4(6):101078. https://doi.org/10.1016/j.xcrm.2023.101078Luo W, Zeng Z, Jin Y, Yang L, Fan T, Wang Z, Pan Y, Yang Y, Yao M, Li Y, Xiao X, Wang G, Wang C, Chang S, Che G, Zhang L, Li Y, Peng Y, Li W. Distinct immune microenvironment of lung adenocarcinoma in never-smokers from smokers. Cell Rep Med. 2023;4(6):101078. https://doi.org/10.1016/j.xcrm.2023.101078

The authors declare that there are no conflicts of interest present.