Experimental faecal peritonitis alters response to 5-hydroxytryptamine in mesenteric lymphatic vessels
https://doi.org/10.23946/2500-0764-2020-5-3-35-41
Abstract
Aim. To compare the reactions to 5-hydroxytryptamine (5-HT, serotonin) in the mesenteric lymphangions upon endothelial denudation and during experimental faecal peritonitis as compared with the control lymphangions.
Materials and Methods. Experiments were performed on the intestinal lymph trunk lymphangions of rats using a pressure myograph system. We examined the changes in contraction frequency and amplitude as well as tonic reactions upon the addition of 5-HT (10-8-10-4 M). Experimental peritonitis was caused by an intraperitoneal injection of feces.
Results. Faecal peritonitis reduced contraction frequency and amplitude in mesenteric lymphangions. 5-HT increased contraction amplitude only at low concentration (10-8 M) and did not alter the tonic reactions. Upon endothelial denudation, serotonin inhibited contraction frequency and amplitude in the lymphangions. As 5-HT stimulates motility through 5-НТ2 receptors and α2-adrenoceptors on endothelial cells, faecal peritonitis abates the sensitivity of such receptors to 5-HT.
Conclusion. In experimental faecal peritonitis, alterations in lymphatic vessels are reminiscent of those in blood vessels. Endothelial dysfunction disturbs the response of lymphatic vessels to 5-HT.
About the Authors
A. A. Egorova
Mechnikov North-Western State Medical University
Russian Federation
Russian Federation
Dr. Alexandra A. Egorova, MD, PhD, Senior Lecturer, Department of Physiology
47/9, Piskarevskiy Prospekt, St. Petersburg, 195067, St. Petersburg
E. A. Avramenko
St. Petersburg State University
Russian Federation
Russian Federation
Dr. Ekaterina A. Avramenko, MD, PhD, Assistant Professor, Department of General Surgery
7-9, Universitetskaya Naberezhnaya, St. Petersburg,199034, St. Petersburg
References
1. Чернядьев С.А., Булаева Э.Б., Кубасов К.А. Патогенетические аспекты развития пареза кишечника при перитоните. Проблемы стоматологии. 2016;12(4):84-89 [Chernyadev SA, Bulaeva EI, Kubasov KA Pathogenetic aspects of the development of intestinal paresis in peritonitis. Actual Problems in Dentistry. 2016;12(4):84-89. (In Russ.).] https://doi.org/10.18481/2077-7566-2016-12-4-84-89
2. Белик Б.М. Оценка клинической эффективности препарата серотонина адипинат в лечении и профилактике синдрома энтеральной недостаточности при распространенном перитоните. Хирургия. Журнал им. Н.И. Пирогова. 2016;9:76- 82 [Belik BM. Evaluation of clinical efficacy of serotonin adipate in treatment and prevention of enteral insufficiency syndrome at generalized peritonitis. Pirogov Russian Journal of Surgery = Khirurgiya. Zhurnal imeni N.I. Pirogova. 2016;9:6-82. (In Russ.).] https://doi.org/10.17116/hirurgia2016976-82
3. Полунина О.С., Воронина Л.П., Полунина Е.А. Севостьянова И.В. Анализ реактивности эндотелия микрососудов кожи при хронической сердечной недостаточности. Регионарное кровообращение и микроциркуляция. 2017;16(4(64)):50-55 [Polunina OS, Voronina LP, Polunina EA, Sevostyanova IV. Analysis of the reactivity of the endothelium of microvessels of the skin in chronic heart failure. Local Microcirculation = Regionarnoe krovoobrashchenie i mikrotsirkulyatsiya. 2017;16(4(64)):50-55. (In Russ.).] https://doi.org/10.24884/1682-6655-2017-16-4-50-55
4. Behringer EJ, Scallan JP, Jafarnejad M, Castorena-Gonzalez JA, Zawieja SD, Moore JE Jr, Davis MJ, Segal SS. Calcium and electrical dynamics in lymphatic endothelium. J Physiol. 2017;595(24):7347-7368. https://doi.org/10.1113/JP274842
5. Liu HN, Hirata H, Okuno Y, Okabe M, Furukawa K. Dopamine and Serotonin Receptors Cooperatively Modulate Pacemaker Activity of Intestinal Cells of Cajal. Chin J Physiol. 2018;61(5):302-312. https://doi.org/10.4077/CJP.2018.BAH607
6. Егорова А.А., Петунов С.Г., Авраменко Е.А. Влияние серотонина на лимфатические сосуды белой крысы. Роль эндотелия. Регионарное кровообращение и микроциркуляция. 2010;1(37):79-82 [Egorova AA, Petunov SG, Avramenko EA Influence of endotelium on the 5-HT effect on lymphatic vessel. Local Microcirculation = Regionarnoe krovoobrashchenie i mikrotsirkulyatsiya. 2010;1(37):79-82. (In Russ.).] https://doi.org/10.24884/1682-6655-2011-10-1-79-82
7. Felsing DE, Canal CE, Booth RG. Ligand-directed serotonin 5-HT2C receptor. Eur J Pharmacol. 2019;848:131-139. https://doi.org/10.1016/j.ejphar.2019.01.037
8. Jiang X, Nicolls MR, Tian W, Rockson SG Lymphatic Dysfunction, Leukotrienes, and Lymphedema. Annu Rev Physiol. 2018;80:49-70. https://doi.org/10.1146/annurev-physiol-022516-034008
9. Кубышкина Н.А., Гайворонская В.В. Эндотоксин-индуцированные изменения функциональной активности лимфатических сосудов. Вестник Санкт-Петербургского университета. Медицина. 2014;(4):205-211 [Kubyshkina NA, Gaivoronskaya VV. Endotoxin induced alterations of functional activity of lymphatic vessels. Vestnik of Saint Petersburg University. Medicine. 2014;(4):205-211. (In Russ.).]
10. Kazimierczyk R, Kamiński K. The role of platelets in the development and progression of pulmonary arterial hypertension. Adv Med Sci. 2018;63(2):312-316. https://doi.org/10.1016/j.advms.2018.04.013
11. Punzi S, Belloli C, Gogny M, Desfontis JC, Mallem MY. Change in vascular smooth muscle response to 5-HT due to short- or long-term endothelial denudation of the bovine digital vein. Vet J. 2016;207:154-159. https://doi.org/10.1016/j.tvjl.2015.10.007
12. Shih CC, Hsu LP, Liao MH, Yang SS, Ho ST, Wu CC. Effects of SPAK on vascular reactivity and nitric oxide production in endotoxemic mice. Eur J Pharmacol. 2017;814:248-254. https://doi.org/10.1016/j.ejphar.2017.08.038
13. Singh AK, Zajdel J, Mirrasekhian E, Almoosawi N, Frisch I, Klawonn AM, Jaarola M, Fritz M, Engblom D. Prostaglandin-mediated inhibition of serotonin signaling controls the affective component of inflammatory pain. J Clin Invest. 2017;127(4):1370-1374. https://doi.org/10.1172/JCI90678
14. Chan AK, von der Weid PY. 5-HT decreases contractile and electrical activities in lymphatic vessels of the guinea-pig mesentery: role of 5-HT 7-receptors. Br J Pharmacol. 2003;139(2):243-254. https://doi.org/10.1038/sj.bjp.0705264
15. McHale NG, Thornbury KD, Hollywood MA. 5-HT inhibits spontaneous contractility of isolated sheep mesenteric lymphatics via activation of 5-HT(4) receptors. Microvasc Res. 2000;60(3):261-268. https://doi.org/10.1006/mvre.2000.2275
Review
For citations:
Egorova A.A., Avramenko E.A. Experimental faecal peritonitis alters response to 5-hydroxytryptamine in mesenteric lymphatic vessels. Fundamental and Clinical Medicine. 2020;5(3):35-41. (In Russ.) https://doi.org/10.23946/2500-0764-2020-5-3-35-41
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