SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTING

Aim. To compare structural and tensile properties of electrospun vascular grafts fabricated of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/ poly(ε-caprolactone) blend and type I collagen solution utilizing either joint or separate feed of these polymers, and to further evaluate adhesion and viability of endothelial cells on both types of the grafts. Materials and Methods. Either 4 mm or 8 mm diameter electrospun grafts were assessed by scanning electron microscopy and uniaxial tension test following culture of human umbilical vein endothelial cells on the graft surface, either Hoechst 33342/PKH26 or acridine orange/ethidium bromide staining, and fluorescence microscopy. Results. Grafts fabricated using the separate feed had poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/ poly(ε-caprolactone) blend and type I collagen located in core and sheath of electrospun fibers, respectively. This two-layer structure of fibers resulted in 5-fold increase in adhesion and 1.5-fold increase in viability of endothelial cells cultured on the graft surface; therefore, the total number of viable endothelial cells increased more than 8-fold. Both types of the grafts demonstrated higher durability and elasticity compared to internal mammary artery, a widely applied vascular conduit. Conclusion. Separate feed of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) blend and type I collagen solution significantly increases the biocompatibility of the electrospun vascular grafts while not affecting their mechanical competence.

сосудистые графты, коллаген I типа, электроспиннинг, биосовместимость, эндотелиальные клетки, vascular grafts, type I collagen, electrospinning, biocompatibility, endothelial cells

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