PRO-INFLAMMATORY PHENOTYPE OF PERIVASCULAR ASTROCYTES AND CD133+ ENDO-THELIAL PROGENITOR CELLS IN MURINE MODEL OF ALZHEIMER’S DISEASE

Aim. To study the expression of NLRP3 in perivascular astrocytes and CD133+ endothelial progenitor cells in mice with amyloid-induced neurodegeneration.Materials and Methods. Ten days following intrahippocampal injection of Aβ1-42, male CD1 mice (4 months, weight 25-30 g) were sacrificed with the further immunohistochemical examination of the NLRP3 expression in S100 beta + and CD133+ cells using confocal microscopy. Results. We detected an increased number of S100beta + cells upon the administration of β-amyloid to the hippocampus. Moreover, a strong colocalization of S100beta and NLRP3 in the dentate gyrus of both groups was revealed indicative of neuroinflammation. CD133 immunostaining did not identify any differences in CD133+ cells between mice with Alzheimer’s disease and sham-treated mice, yet there was a notable reduction in CD133+ cell count after intrahippocampal injection of β-amyloid. Conclusion. Neuroinflammation induced by β-amyloid is associated with the formation of NL-RP3 inflammasomes and decreased neovascularization capacity.

периваскулярная астроглия, инфламмасомы NLRP3, клетки-предшественники эндотелиоцитов, моделирование болезни Альцгеймера у мышей, perivascular astrocytes, NLRP3 inflammasomes, endothelial progenitor cells, Alzheimer's disease

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