Morphological and chemical characterization of magnesium phosphate and calcium phosphate bions
D. K. Shishkova,
T. V. Glushkova,
O. S. Efimova,
A. N. Popova,
V. Yu. Malysheva,
R. P. Kolmykov,
Z. R. Ismagilov,
A. K. Gutakovsky,
Yu. A. Zhivodkov,
A. S. Kozhukhov,
O. G. Sevostyanov,
V. F. Dolganyuk,
Y. А. Kudryavtseva,
A. G. Kutikhin https://doi.org/10.23946/2500-0764-2019-4-2-6-16
Abstract
Aim. To compare morphological properties, mineral, and organic pattern of magnesium phosphate bions (MPB) and calcium phosphate bions (CPB) for the assessment of MPB as a control group for the evaluation of СРВ-specific endothelial toxicity.
Materials and Methods. Both MPB and CPB were artificially synthesized employing blood-mimetic medium supersaturated of magnesium and phosphorus salts, respectively. Morphology of MPB and CPB was investigated by electron and atomic force microscopy, elemental analysis was performed utilizing energy-dispersive X-ray spectroscopy, atomic emission spectroscopy, and CHN-SO analysis, functional groups were examined using Fourier-transform infrared spectroscopy and Raman spectroscopy, while chemical formula was identified by X-ray powder diffraction analysis. Protein profile of MPB and СРВ was screened employing polyacrylamide gel electrophoresis with the following silver staining.
Results. Both MPB and CPB represented spherical spongeous particles of 80-200 nm diameter and mean diameter of around 120 nm, tending to form clusters of several particles. Both MPB and CPB contained carbon, oxygen, nitrogen, hydrogen, and phosphorus. However, MPB also contained magnesium but had minimum calcium content while СРВ had a significant amount of calcium but were devoid of magnesium. Both MPB and CPB contained phosphate, carbonate, and hydroxyl functional groups but MPB consisted of magnesium phosphate hydrate and huntite while CPB were comprised of hydroxyapatite and carbonate-hydroxyapatite. Protein composition of MPB and СРВ was similar.
Conclusion. MPB are similar to CPB excepting the chemical formula; hence, MPB can be considered as an appropriate control group to evaluate specificity of СРВ-related endothelial toxicity.
Keywords
nanoparticles,
bions,
toxicity,
endothelium,
atherosclerosis,
elemental analysis,
mineral profile,
organic profile
Supporting agencies: The reported study was funded by Russian Foundation for Basic Research according to the research project № 17-04-00570
About the Authors
D. K. Shishkova
Research Institute for Complex Issues of Cardiovascular Diseases
Russian Federation
Russian Federation
Daria К. Shishkova - MSc, Junior Researcher, Laboratory for Vascular Biology, Division ofExperimental and Clinical Cardiology.
6, Sosnovy Boulevard, Kemerovo, 650002
T. V. Glushkova
Research Institute for Complex Issues of Cardiovascular Diseases
Russian Federation
Russian Federation
Tatiana V. Glushkova - Dr., PhD, Researcher, Laboratory for Novel Biomaterials, Division ofExperimental and Clinical Cardiology.
6, Sosnovy Boulevard, Kemerovo, 650002
O. S. Efimova
Institute of Coal Chemistry and Material Science, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Olga S. Efimova - Dr., PhD, Researcher, Laboratory for High Temperature Carbon Materials.
18, Sovietskiy Avenue, Kemerovo, 650000
A. N. Popova
Institute of Coal Chemistry and Material Science, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Anna N. Popova - Dr., PhD, Senior Researcher, Laboratory for Inorganic Nanomaterials.
18, Sovietskiy Avenue, Kemerovo, 650000
V. Yu. Malysheva
Institute of Coal Chemistry and Material Science, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Valentina Yu. Malysheva - MSc, Leading Technologist, Laboratory for High Temperature Carbon Materials.
18, Sovietskiy Avenue, Kemerovo, 650000
R. P. Kolmykov
Institute of Coal Chemistry and Material Science, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Roman P. Kolmykov - Dr., PhD, Senior Researcher, Laboratory for Inorganic Nanomaterials.
18, Sovietskiy Avenue, Kemerovo, 650000
Z. R. Ismagilov
Institute of Coal Chemistry and Material Science, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Zinfer R. Ismagilov - Prof., DSc, Corresponding Member of the Russian Academy of Sciences, Head.
18, Sovietskiy Avenue, Kemerovo, 650000
A. K. Gutakovsky
Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Anton K. Gutakovsky - Dr., PhD, Leading Researcher, Laboratory ofNanodiagnostics and Nanolitography, Department ofPhysics and Technology ofLow-Dimensional Semiconductors.
13, Prospekt Lavrentieva, Novosibirsk, 630090
Yu. A. Zhivodkov
Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Yuriy A. Zhivodkov - MSc, Leading Engineer-Technologist, Laboratory ofNanodiagnostics andNanolitography, Department ofPhysics and Technology ofLow-Dimensional Semiconductors, Micro- and Nanostructures.
13, Prospekt Lavrentieva, Novosibirsk, 630090
A. S. Kozhukhov
Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Anton S. Kozhukhov - MSc, PhD Student, Laboratory of Nanodiagnostics andNanolitography, DepartmentofPhysics and Technology ofLow-Dimensional Semiconductors.
13, Prospekt Lavrentieva, Novosibirsk, 630090
O. G. Sevostyanov
Kemerovo State University
Russian Federation
Russian Federation
Oleg G. Sevostyanov - Dr., PhD, Associate Professor, Department of Experimental Physics.
6, Krasnaya Street, Kemerovo, 650000
V. F. Dolganyuk
Kemerovo State University
Russian Federation
Russian Federation
Viatcheslav F. Dolganyuk - Dr., PhD, Senior Researcher, Research Institute for Biotechnology.
6, Krasnaya Street, Kemerovo, 650000
Y. А. Kudryavtseva
Research Institute for Complex Issues of Cardiovascular Diseases
Russian Federation
Russian Federation
6, Sosnovy Boulevard, Kemerovo, 650002
A. G. Kutikhin
Research Institute for Complex Issues of Cardiovascular Diseases
Russian Federation
Russian Federation
Anton G. Kutikhin - Dr., MD, PhD, Head of the Laboratory for Vascular Biology, Division ofExperimental and Clinical Cardiology.
6, Sosnovy Boulevard, Kemerovo, 650002
References
1. Wu CY, Young L, Young D, Martel J, Young JD. Bions: a family of biomimetic mineralo-organic complexes derived from biological fluids. PLoS One. 2013; 8 (9): e75501. doi: 10.1371/journal.pone.0075501.
2. Kutikhin AG, Velikanova EA, Mukhamadiyarov RA, Glushkova TV, Borisov VV, Matveeva VG, et al. Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions. Sci Rep. 2016; 6: 27255. doi: 10.1038/srep27255.
3. Molenaar FM, van Reekum FE, Rookmaaker MB, Abrahams AC, van Jaarsveld BC. Extraosseous calcification in end-stage renal disease: from visceral organs to vasculature. Semin Dial. 2014; 27 (5): 477-487. doi: 10.1111/sdi.l2177.
4. Nigwekar SU, Kroshinsky D, Nazarian RM, Goverman J, Malhotra R, Jackson VA, et al. Calciphylaxis: risk factors, diagnosis, and treatment. Am J Kidney Dis. 2015; 66 (1): 133146. doi: 10.1053/j.ajkd.2015.01.034.
5. Yurdagul A Jr, Finney AC, Woolard MD, Orr AW. The arterial microenvironment: the where and why of atherosclerosis. Biochem J. 2016; 473 (10): 1281-1295. doi: 10.1042/BJ20150844.
6. Gimbrone MA Jr, Garcia-Cardena G. Endothelial Cell Dysfunction and the Pathobiology of Atherosclerosis. Circ Res. 2016; 118 (4): 620-636. doi: 10.1161/CIRCRESAHA.115.306301.
7. GBD 2016 Causes of Death Collaborators. Global, regional, and national age-sex specific mortality for 264 causes of death, 1980-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017; 390 (10100): 1151-1210. doi: 10.1016/S0140-6736(17)32152-9.
8. Jensen HA, Mehta JL. Endothelial cell dysfunction as a novel therapeutic target in atherosclerosis. Expert Rev Cardiovasc Ther. 2016; 14 (9): 1021-1033. doi: 10.1080/14779072.2016.1207527.
9. Cahill PA, Redmond EM. Vascular endothelium - Gatekeeper of vessel health. Atherosclerosis. 2016; 248: 97-109. doi: 10.1016/j.atherosclerosis.2016.03.007.
10. Blau R, Krivitsky A, Epshtein Y, Satchi-Fainaro R. Are nanotheranostics and nanodiagnostics-guided drug delivery stepping stones towards precision medicine? Drug Resist Updat. 2016; 27: 39-58. doi: 10.1016/j.drup.2016.06.003.
11. Matea CT, Mocan T, Tabaran F, Pop T, Mosteanu O, Puia C, et al. Quantum dots in imaging, drug delivery and sensor applications. Int J Nanomedicine. 2017; 12: 5421-5431. doi: 10.2147/IJN.S138624.
12. Peng HH, Wu CY, Young D, Martel J, Young A, Ojcius DM, et al. Physicochemical and biological properties of biomimetic mineralo-protein nanoparticles formed spontaneously in biological fluids. Small. 2013; 9 (13): 2297-2307. doi: 10.1002/smll.201202270.
13. Young JD, Martel J, Young L, Wu CY, Young A, Young D. Putative nanobacteria represent physiological remnants and culture by-products of normal calcium homeostasis. PLoS One. 2009; 4 (2): e4417. doi: 10.1371/journal.pone.0004417.
14. Martel J, Young D, Young A, Wu CY, Chen CD, Yu JS, et al. Comprehensive proteomic analysis of mineral nanoparticles derived from human body fluids and analyzed by liquid chromatography-tandem mass spectrometry. Anal Biochem. 2011; 418 (1): 111-125. doi: 10.1016/j.ab.2011.06.018.
15. Smith ER, Hanssen E, McMahon LP, Holt SG. Fetuin-A-containing calciprotein particles reduce mineral stress in the macrophage. PLoS One. 2013; 8 (4): e60904. doi: 10.1371/journal.pone.0060904.
Review
For citations:
Shishkova D.K., Glushkova T.V., Efimova O.S., Popova A.N., Malysheva V.Yu., Kolmykov R.P., Ismagilov Z.R., Gutakovsky A.K., Zhivodkov Yu.A., Kozhukhov A.S., Sevostyanov O.G., Dolganyuk V.F., Kudryavtseva Y.А., Kutikhin A.G. Morphological and chemical characterization of magnesium phosphate and calcium phosphate bions. Fundamental and Clinical Medicine. 2019;4(2):6-16. (In Russ.) https://doi.org/10.23946/2500-0764-2019-4-2-6-16
Views:
589
Email this article 