Chromatography of Hedysarum extracts on modified Sepharose CL sorbents
https://doi.org/10.23946/2500-0764-2019-4-3-61-67
Abstract
Aim. To study the chromatographic efficiency of modified Sepharose CL-4B sorbents for separation, purification, and preparation of major components from Hedysarum theinum Krasnob. and Hedysarum neglectum Ledeb. root extracts.
Materials and Methods. Sorption capacity of the native and modified sepharose was evaluated in both isocratic and gradient elution modes. 4-pyridinecarboxylic acid derivative [(4-hydroxy-3-methoxyphenyl)methylene]hydrazide (ftivazide) was used as a sorbent modifier ligand. We studied chromatographic properties of Sepharose CL-4B, Sepharose CL-6B, and azoepoxyadsorbent Sepharose CL-4B-DEP-p-nitrobenzohydrazide-ftivazide, a chemically modified analogue of Sepharose CL-4B.
Results. Sepharose CL-4B and Sepharose CL-4B-DEP-p-nitrobenzohydrazide-ftivazide were found efficient carriers for liquid column chromatography of water-ethanol Hedysarum extracts. Complete elution of bioactive substances has been achieved exclusively by using sodium tetraborate as an eluent. We were able to identify flavonoids and tannins of different condensation in chromatographic fractions.
Conclusion. Comparative chromatography of bioactive substances from Hedysarum root extracts is more efficient if employing Sepharose CL-4B instead of Sepharose CL-6B as a sorbent. Sepharose CL-4B-DEP-p-nitrobenzohydrazide-ftivazide demonstrates excellent separation capacity for tannins and flavonoids.
About the Authors
Yu. S. Fedorova
Kemerovo State Medical University
Russian Federation
Russian Federation
MD, PhD, Associate Professor, Department of Pharmaceutical and General Chemistry,
22a, Voroshilova Street, Kemerovo, 650056
A. S. Sukhikh
Kemerovo State Medical University
Russian Federation
Russian Federation
MD, PhD, Senior Researcher, Central Research Laboratory,
22a, Voroshilova Street, Kemerovo, 650056
N. I. Suslov
Siberian State Medical University;
Goldberg Research Institute of Pharmacology and Regenerative Medicine
Russian Federation
Russian Federation
MD, DSc, Professor, Head of the Laboratory for Phytopharmacology and Special Nutrition,
2, Moskovskiy Trakt, Tomsk, 634050
3, Lenina Prospekt, 634028, Tomsk
Yu. V. Zakhar
Kemerovo State Medical University
Russian Federation
Russian Federation
MD, PhD, Associate Professor, Department of Microbiology, Immunology and Virology,
22a, Voroshilova Street, Kemerovo, 650056
O. M. Soboleva
Kemerovo State Medical University
Russian Federation
Russian Federation
PhD, Associate Professor, Department of Microbiology, Immunology and Virology,
22a, Voroshilova Street, Kemerovo, 650056
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Review
For citations:
Fedorova Yu.S., Sukhikh A.S., Suslov N.I., Zakhar Yu.V., Soboleva O.M. Chromatography of Hedysarum extracts on modified Sepharose CL sorbents. Fundamental and Clinical Medicine. 2019;4(3):61-67. (In Russ.) https://doi.org/10.23946/2500-0764-2019-4-3-61-67
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