ASSESSMENT OF CRITICAL BUTYRIC ACID CONCENTRATIONS IN FECES OF PATIENTS ON ENTERAL TUBE FEEDING IN INTENSIVE CARE UNITS
Abstract
Aim. To determine the critical butyric acid concentrations in the feces of patients on enteral tube feeding. Materials and Methods. We investigated oropharyngeal and gut microbiota of 31 patients admitted to intensive care unit and further supplied with enteral tube feeding. Quantication of microorganisms was performed by seeding into liquid agar medium. Activity of oropharyngeal and gut microbiota was determined by measuring concentrations of short-chain fatty acids using gas-liquid chromatography. Results. At the fecal butyric acid concentration of 2.2 mmol/g, a ratio of fecal levels of acetic, propionic, and butyric acids (C2:C3:C4) was 72:17:11, with a structural index of 0.56. Below fecal butyric acid concentration of 2.2 mmol/g, a proportion of acetic acid decreased linearly, with a decrease in structural index. Conclusions. Decrease in fecal butyric acid concentration < 2.2 mmol/g impairs activity of gut microbiota and induces further metabolic changes in the gut.
About the Authors
ALEKSANDR M. Zatevalov
G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
VLADIMIR A. Aleshkin
G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
EVGENIYA P. Sel'kova
G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
TATYANA A. Grenkova
G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
References
1. Алёшкин В.А., Афанасьев С.С., Караулов А.В., Воропаева Е.А., Афанасьев М.С., Алёшкин А.В. с соавт. Микробиоценозы и здоровье человека: монография / под общ. ред. Алёшкина В.А., Афанасьева С.С., Караулова А.В. Москва: Династия, 2015. 547 c.
2. Holmes E, Li JV, Athanasiou T, Ashraan H, Nicholson JK. Understanding the role of gut microbiome-host metabolic signal disruption in health and disease. Trends Microbiol. 2011; 19 (7): 349-359.
3. Peris-Bondia F, Latorre A, Artacho A, Moya A, D’Auria G. The active human gut microbiota differs from the total microbiota. PLoS One. 2011; 6 (7): e22448.
4. Thornton DJ, Rousseau K, McGuckin MA. Structure and function of the polymeric mecins in airways mucus. Annu. Rev. Physiol. 2008; 70: 459-486.
5. Ахременко Я.А. Бочкарева О.П., Красноженов Е.П. Колонизационная резистентность - универсальный механизм противоинфекционной защиты // Проблемы современной науки. 2013. № 7-2. С.104-115.
6. Hattori M, Taylor TD. The human intestinal microbiome: a new frontier of human biology. DNA Research. 2009; 16 (1): 1-12.
7. Shenderov BA. Probiotics and functional foods. In Food Engineering, Encyclopedia of Life Support Systems (EOLSS). Developed under the Auspices of the UNESCO, Eolss Publishers, Oxford, UK. - 33. at: http:www.eolss.net.
8. Shenderov BA. Probiotic (symbiotic) bacterial languages. Anaerobe. 2011; 17 (6): 490-495.
9. Марри Р., Греннер Д., Мейес П., Родуэлл В. Биохимия человека, пер. с англ., в 2 т. М.: Мир, 2009. 800 с.
10. Defoirdt T, Boon N, Sorgeloos P, Verstraete W, Bossier P. Short-chain fatty acids and poly-beta-hydroxyalkanoates: (New) Biocontrol agents for a sustainable animal production. Biotechnol. Advances. 2009; 27 (6): 680-685.
11. Steinmann J, Halldórsson S, Agerberth B, Gudmundsson GH. Phenylbutyrate induces antimicrobial peptide expression. Antimicrob. Agents Chemother. 2009; 53 (12): 5127-5133
12. Затевалов А.М. Киселева И.А., Копанев Ю.А., Алешкин А.В., Афанасьев С.С., Селькова Е.П. Влияние бактериофагов на микрофлору толстой кишки // Материалы 1 международной научно-практической конференции «Бактериофаги: теоретические и практические аспекты применения в медицине, ветеринарии и пищевой промышленности». Ульяновск: УГСХА им. П.А. Столыпина. 2013. Т.2. С.9-14.
13. Головенко О.В, Халиф И.Л., Головенко А.О. Роль масляной кислоты в лечении органических и функциональных заболеваний толстой кишки (обзор литературы) // Клинические перспективы гастроэнтерологии, гепатологии. 2011. №3. С. 27-36.
14. Neish AS. Microbes in gastrointestinal health and disease. Gastroenterology. 2009; 136 (1): 65-80.
15. Ng SC, Hart AL, Kamm MA, Stagg AJ, Knight SC. Mechanisms of action of probiotics: recent advances. Inamm Bowel Dis. 2009; 15 (2): 300-310.
16. Ruemmele FM, Bier D, Marteau P, Rechkemmer G, Bourdet-Sicard R, Walker WA et al. Clinical evidence for immunomodulatory effects of probiotic bacteria. J. Pediatr. Gastroenterol. Nutr. 2009; 48 (2): 126-141.
17. Алёшкин В.А., Селькова Е.П., Затевалов А.М., Миронов А.Ю., Волчецкий А.Л., Гудова Н.В. Определение дисбиотических изменений желудочно-кишечного тракта по маркёрам содержимого кишечника. Федеральные клинические рекомендации. - Н.Новгород: Изд-во «Ремедиум Приволжье», 2016. - 40 с.
Review
For citations:
Zatevalov A.M., Aleshkin V.A., Sel'kova E.P., Grenkova T.A. ASSESSMENT OF CRITICAL BUTYRIC ACID CONCENTRATIONS IN FECES OF PATIENTS ON ENTERAL TUBE FEEDING IN INTENSIVE CARE UNITS. Fundamental and Clinical Medicine. 2017;2(1):14-22. (In Russ.)
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