Mouse hippocampal neurospheres negatively regulate cerebral angiogenesis
https://doi.org/10.23946/2500-0764-2020-5-3-18-23
Abstract
Aim. To evaluate the features of cerebral angiogenesis in the presence of neurospheres, free-floating clusters of neural stem and progenitor cells.
Materials and Methods. Endothelial cells were optionally co-cultured with neurospheres were isolated from the hippocampus of 10-14-days-old C57Bl6 mice. Angiogenesis was evaluated in vitro by means of the angiogenesis assay kit (Abcam, ab204726) according to the manufacturer's protocol.
Results. The number of vascular loops was decreased in the presence of neurospheres. Other angiogenic parameters (average loop area and perimeter, total vessel length and number of contacts per mm2) had no statistically significant differences after 18 hours of culture.
Conclusion. The proliferative activity of neurospheres inhibits in vitro cerebral angiogenesis.
Supporting agencies: The study was supported by the Ministry of Health of the Russian Federation (State Assignment for Research, 2018-2020).
About the Authors
Yu. A. Uspenskaya
Research Institute of Molecular Medicine and Pathological Biochemistry, Voino-Yasenetskiy Krasnoyarsk State Medical University; Krasnoyarsk State Agrarian University
Russian Federation
Russian Federation
Prof. Yulia A. Uspenskaya, DSc, Associate Professor, Research Fellow, Research Institute of Molecular Medicine and Pathological Biochemistry; Professor, Department of Internal Noncommunicable Diseases, Obstetrics and Physiology of Livestock
1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022
90, Prospekt Mira, Krasnoyarsk, 660049
N. A. Malinovskaya
Research Institute of Molecular Medicine and Pathological Biochemistry, Voino-Yasenetskiy Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Prof. Natalia A. Malinovskaya, MD, DSc, Senior Researcher, Research Institute of Molecular Medicine and Pathological Biochemistry
1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022
A. V. Morgun
Research Institute of Molecular Medicine and Pathological Biochemistry, Voino-Yasenetskiy Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Dr. Andrey V. Morgun, MD, DSc, Leading Researcher, Research Institute of Molecular Medicine and Pathological Biochemistry
1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022
E. D. Osipova
Research Institute of Molecular Medicine and Pathological Biochemistry, Voino-Yasenetskiy Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Mrs. Elena D. Osipova, Research Fellow, Research Institute of Molecular Medicine and Pathological Biochemistry
1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022
A. B. Salmina
Research Institute of Molecular Medicine and Pathological Biochemistry, Voino-Yasenetskiy Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Prof. Alla B. Salmina, MD, DSc, Head of the Research Institute of Molecular Medicine and Pathological Biochemistry, Head of the Department of Biological, Medical, Pharmaceutical, and Toxicological Chemistry
1, Partizana Zheleznyaka Street, Krasnoyarsk, 660022
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Review
For citations:
Uspenskaya Yu.A., Malinovskaya N.A., Morgun A.V., Osipova E.D., Salmina A.B. Mouse hippocampal neurospheres negatively regulate cerebral angiogenesis. Fundamental and Clinical Medicine. 2020;5(3):18-23. (In Russ.) https://doi.org/10.23946/2500-0764-2020-5-3-18-23
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