Gene-specific features of tick-borne borreliosis in Russia
https://doi.org/10.23946/2500-0764-2021-6-3-94-99
Abstract
Aim. To study the gene-specific composition of Borrelia spp. in ixodid ticks inhabiting the foci of ixodid tick-borne borreliosis (ITBB) in the south of Western Siberia.
Materials and Methods. The gene-specific composition of Borrelia spp. in ixodid ticks was determined by inoculation on a BSK-H nutrient medium, real-time polymerase chain reaction, and sequencing (1148 specimens of ixodid ticks collected from vegetation and 2183 specimens withdrawn from humans).
Results. Infection of ticks with borrelia ranged from 22.4% in the Altai Republic to 56.9% in the Novosibirsk Region. There were no significant differences in the levels of Borrelia infection between two major ixodides, I. persulcatus and I. pavlovskiy (average infection levels 40.0% and 38.8%, respectively). At least five gene species of pathogenic Borrelia (B. garinii, B. afzelii, B. bavariensis, B. miyamotoi, and B. spielmanii) have been identified. The GenBank database contains 45 nucleotide sequences of the intergenic spacer rrf (5S)-rrl (23S). Prevalence of gene species B. garinii and B. afzelii in different ticks (I. persulcatus and I. pavlovskiy) did not differ significantly, yet B. garinii was found to be more frequent than B. afzelii. The frequency of infection of I. persulcatus ticks with B. miyamotoi was significantly (3.5-fold) as compared to those of B. garinii and B. afzelii. In D. reticulatus ticks, the DNA of B. spielmanii and B. miyamotoi was detected.
Conclusion. It is necessary to continue studies to assess the role of the meadow ticks D. reticulatus in the circulation of different Borrelia spp. in various natural foci within the Russian Federation.
About the Authors
S. A. Rudakova
Omsk Research Institute of Natural Focal Infections
Russian Federation
Russian Federation
Svetlana A. Rudakova, Dr., MD, DSc, Head of the Laboratory for Molecular Diagnostics with a Group of Tick-Borne Borrelioses, Chief Scientific Officer; Head of the Department of Zoonotic Infections
7, Mira Prospekt, Omsk, 644080
N. V. Rudakov
Omsk Research Institute of Natural Focal Infections;
Omsk State Medical University
Russian Federation
Russian Federation
Nikolay V. Rudakov, Prof., MD, DSc, Director, Omsk Research Institute of Natural Focal Infections; Head of the Department of Microbiology, Virology and Immunology, Omsk State Medical University
7, Mira Prospekt, Omsk, 644080,
12, Lenina Street, Omsk
S. V. Shtrek
Omsk Research Institute of Natural Focal Infections;
Omsk State Medical University
Russian Federation
Russian Federation
Sergey V. Shtrek, Dr., MD, PhD, Senior Researcher, Laboratory of Zoonotic Infections, Omsk Research Institute of Natural Focal Infections; Assistant Professor, Department of Microbiology, Virology and Immunology, Omsk State Medical University, Omsk, Russian Federation
7, Mira Prospekt, Omsk, 644080,
12, Lenina Street, Omsk
O. E. Teslova
Omsk Research Institute of Natural Focal Infections;
Omsk State Medical University
Russian Federation
Russian Federation
Olga E. Teslova, Dr., MD, Junior Researcher, Laboratory for Molecular Diagnostics with a Group of Tick-Borne Borrelioses, Omsk Research Institute of Natural Focal Infections; Assistant Professor, Department of Microbiology, Virology and Immunology, Omsk State Medical University, Omsk, Russian Federation
7, Mira Prospekt, Omsk, 644080,
12, Lenina Street, Omsk
N. E. Kaneshova
Omsk Research Institute of Natural Focal Infections;
Omsk State Medical University
Russian Federation
Russian Federation
Nadia E. Kaneshova, Dr., MD, Junior Researcher, Laboratory for Molecular Diagnostics with a Group of Tick-Borne Borrelioses, Omsk Research Institute of Natural Focal Infections; Assistant Professor, Department of Microbiology, Virology and Immunology, Omsk State Medical University, Omsk, Russian Federation
7, Mira Prospekt, Omsk, 644080,
12, Lenina Street, Omsk
References
1. Rudakov NV, Rudakova SA. Tick-borne infections in Siberia. Practical guide. Omsk : Izdatel'skiy tsentr «Omskiy nauchnyy vestnik»; 2019. (In Russ).
2. Korenberg EI, Pomelova VG, Osin NS. Infections with natural focality transmitted by Ixodid ticks. Moscow; 2013. (In Russ).
3. O sostoyanii sanitarno-epidemiologicheskogo blagopoluchiya naseleniya v Rossiyskoy Federatsii v 2018 godu: Gosudarstvennyy doklad. Moscow: Federal'naya sluzhba po nadzoru v sfere zashchity prav potrebiteley i blagopoluchiya cheloveka; 2019. (In Russ). Available at: https://www.rospotrebnadzor.ru/upload/iblock/798/gosudarstvennyy-doklad-o-sostoyanii-sanitarno_epidemiologicheskogo-blagopoluchiya-naseleniya-v-rossiyskoy-federatsii-v-2018-godu.pdf. Accessed: 24 August, 2021.
4. Karan' LS, Kolyasnikova NM, Sarksyan DS, Gridneva KA, Fedorova MV, Esaulkova AYu, Romanenko VV. Infitsirovannost' iksodovykh kleshchey i melkikh mlekopitayushchikh borreliyami razlichnykh taksonomicheskikh grupp. Molekulyarnaya diagnostika. 2014:493-494. (In Russ).
5. Fukunaga M, Takahashi Y, Tsuruta Y, Matsushita O, Ralph D, McClelland M, Nakao M. Genetic and phenotypic analysis of Borrelia miyamotoi sp. nov., isolated from the ixodid tick Ixodes persulcatus, the vector for Lyme disease in Japan. Int J Syst Bacteriol. 1995;45(4):804-810. https://doi.org/10.1099/00207713-45-4-804. PMID: 7547303
6. Mukhacheva TA, Salikhova II, Kovalev SY. Multilocus spacer analysis revealed highly homogeneous genetic background of asian type of Borrelia miyamotoi. Infect Genet Evol. 2015;31:257-262. https://doi.org/10.1016/j.meegid.2015.02.009
7. Sinski E, Welc-Faleciak R, Zajkowska J. Borrelia miyamotoi: A human tick-borne relapsing fever spirochete in Europe and its potential impact on public health. Adv Med Sci. 2016;61(2):255-260. https://doi.org/10.1016/j.advms.2016.03.001
8. FGBU NIIDI FMBA Rossii, Obshchestvennaya organizatsiya «Evroaziatskoe obshchestvo po infektsionnym boleznyam», Obshchestvennaya organizatsiya «Assotsiatsiya vrachey infektsionistov Sankt-Peterburga i Leningradskoy oblasti» (AVISPO). Klinicheskie rekomendatsii (protokol lecheniya) okazaniya meditsinskoy pomoshchi detyam bol'nym bolezn'yu Layma. Saint Petersburg; 2015. (In Russ). Available at: http://niidi.ru/dotAsset/aacc042d-345c-4b45-9a4b-fa9d0d9e6714.pdf. Accessed: 24 August, 2021.
9. Platonov AE, Koetsveld J, Kolyasnikova NM, Sarksyan DS, Toporkova MG, Shipulin GA, Hovius JW. Microbiological evidence of etiology "ixodes tick-borne borreliosis without erythema migrans" - infection caused by borrelia miyamotoi. Epidemiology and vaccinal prevention. 2017;16(1):29-35. (In Russ). https://doi.org/10.31631/2073-3046-2017-16-1-29-35
10. Fadeeva IA, Nefedova VV, Korenberg E, Gorelava NB. Genetic variants of borrelia afzeli, a path-ogen of the ixodes tick borrelioses. Molekularnaya genetika mikrobiologia i virusologia. 2005;3:18-21. (In Russ).
11. Nefedova VV, Korenberg EI, Gorelova NB. Genetic variants of borrelia garinii, a widespread eurasian pathogen of ixodic tick borreliosis. Molecular Genetics, Microbiology and Virology. 2010;25(3):95-100. (In Russ).
12. Nefedova VV, Korenberg EI, Gorelova NB. Multilocus sequence analysis of "atypical" borrelia burgdorferi sensu lato isolated in Russia. Molecular Genetics, Microbiology and Virology. 2017;32(4):196-203. (In Russ). https://doi.org/10.3103/S0891416817040073
13. Korenberg EI, Sirotkin MB, Kovalevskii YuV. A general scheme of the circulation of ixodid tick-borne borrelioses pathogens in natural foci of Eurasia. Zoologicheskiy zhurnal. 2016;95(3):283-299. (In Russ). https://doi.org/10.7868/S0044513416030090
14. Moskvitina NS, Korobitsyn IG, Tyutenkov OYu, Gashkov SI, Kononova YuV, Moskvitin SS, Romanenko VN, Mikryukova TP, Protopopova EV, Kartashov MYu, Chausov EV, Konovalova SN, Tupota NL, Sementsova AO, Ternovoy VA, Loktev VB. Possible role of migratory birds in the spread tick-borne infections in the territory of Siberia and Russian far east. Achievements in the Life Sciences.2014;9:127-131. (In Russ).
15. Rudakova SA. Molekulyarno-epidemiologicheskiy monitoring za infektsiyami, peredayushchimisya iksodovymi kleshchami v sochetannykh prirodnykh ochagakh. Molekulyarnaya diagnostika: sbornik trudov, Moscow, 18-20 marta 2014 goda. Moscow: Izdatel'stvo MBA, 2014:499-500. (In Russ).
16. Rudakova SA, Teslova OE, Kaneshova NE, Shtrek SV, Yakimenko VV, Penyevskaya NA. Genospecies diversity of borrelia in ixodes ticks of the west Siberia. Problems of particularly dangerous infections. 2019;(4):92- 96. (In Russ). https://doi.org/10.21055/0370-1069-2019-4-92-96
17. Bondarenko EI, Leonova GN, Shchuchinova LD, Shchuchinov LV, Sukhoverkova AV, Ivanov LI, Mzhel'skaya TV, Dragomeretskaya AG, Mokretsova EV, Andaev EI, Trushina YuN, Zvereva NG, Timofeev DI, Ofitserov VI. Rasprostranennost' Borellia miyamotoi – vozbuditelya kleshchevoy vozvratnoy likhoradki v semi regionakh Sibiri i Dal'nego Vostoka. Molekulyarnaya diagnostika 2017, Moscow, 18-20 aprelya 2017 goda. 2017:168-170. (In Russ).
18. Sarksian DS. Ixodes tick borreliosis - current state of problem. Infectious diseases. 2015;2(13):61-67. (In Russ).
19. Rar V, Fomenko N, Dobrotvorsky A, Livanova N, Rudakova S, Fedorov E, Astanin V, Morozova O. Tickborne Pathogen Detection, Western Siberia, Russia. Emerg Infect Dis. 2005;11(11):1708-1715. https://doi.org/10.3201/eid1111.041195
20. Rudakova SA, Fomenko NV, Tupikin AE, Livanova NN. Rezul'taty genotipirovaniya borreliy v kleshchakh Dermacentor reticulatus v Zapadnoy Sibiri. Meditsinskaya mikrobiologiya – XXI vek. Saratov; 2004:196-197. (In Russ).
Review
For citations:
Rudakova S.A., Rudakov N.V., Shtrek S.V., Teslova O.E., Kaneshova N.E. Gene-specific features of tick-borne borreliosis in Russia. Fundamental and Clinical Medicine. 2021;6(3):94-99. (In Russ.) https://doi.org/10.23946/2500-0764-2021-6-3-94-99
Views:
535
Email this article 