MASS SPECTROMETRY OF PROTEINS EXTRACTED FROM PLAQUE-DERIVED CALCIUM PHOSPHATE BIONS
Abstract
Aim. To investigate the proteomic profile of calcium phosphate bions (CPB) derived from atherosclerotic plaques of large human arteries. Materials and Methods. CPB were extracted from femoral atherosclerotic plaques of two patients with coronary artery disease and significant peripheral atherosclerosis utilizing an original protocol. After sodium dodecyl sulfate polyacrylamide gel electrophoresis, we performed tryptic hydrolysis followed by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Mass spectra were identified using the Mascot search engine. Results. Plaque-derived CPB contained bovine and human serum albumin, bovine alpha-2-macroglobulin, and human cytoplasmic actin 1. Hence, we showed that CPB adsorb proteins from both fetal bovine serum and atherosclerotic plaques. Conclusion. Plaque-derived CPB have similar proteomic profile to artificially synthesized CPB, yet adsorbing plaque proteins in addition to those characteristic for the serum.
About the Authors
ARSENIY E. Yuzhalin
CRUK/MRC Oxford Institute for Radiation Oncology
Russian Federation
Russian Federation
EVGENIY A. Demidov
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
SERGEY E. Peltek
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
References
1. Heiss A, Pipich V, Jahnen-Dechent W, Schwahn D. Fetuin-A is a mineral carrier protein: small angle neutron scattering provides new insight on Fetuin-A controlled calcification inhibition. Biophys J. 2010; 99 (12): 3986-3995.
2. Wu CY, Young L, Young D, Martel J, Young JD. Bions: a family of biomimetic mineralo-organic complexes derived from biological fluids. PLoS One. 2013; 8 (9): e75501.
3. Kutikhin AG, Velikanova EA, Mukhamadiyarov RA, Glushkova TV, Borisov VV, Matveeva VG., et al. Apoptosismediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions. Sci Rep. 2016; (6): 27255.
4. Larance M, Lamond AI. Multidimensional proteomics for cell biology. Nat Rev Mol Cell Biol. 2015; 16 (5): 269-280.
5. Altelaar AF, Munoz J, Heck AJ. Next-generation proteomics:towards an integrative view of proteome dynamics. Nat Rev Genet. 2013; 14 (1): 35-48.
Review
For citations:
Yuzhalin A.E., Demidov E.A., Peltek S.E. MASS SPECTROMETRY OF PROTEINS EXTRACTED FROM PLAQUE-DERIVED CALCIUM PHOSPHATE BIONS. Fundamental and Clinical Medicine. 2018;3(2):12-19. (In Russ.)
Views:
397
Email this article 