PREVALENCE AND TYPES OF AZF MICRODELETIONS IN INFERTILE MEN
Abstract
Aim. To investigate prevalence and types of azoospermia factor (AZF) microdeletions in infertile men.Materials and Methods. We performed the genotyping of AZF locus within Y chromosome in 76 male inhabitants of Kemerovo Region (23-38 years of age, mean with standard error 34.2 ± 2.35) with primary infertility. DNA was extracted from the peripheral blood utilizing thermocoagulation method with the following amplication of sequence tagged sites (STS) specic for AZFa, AZFb, and AZFc subregions. We calculated the prevalence of AZF microdeletions and proportions of their different types.Results. The prevalence of AZF microdeletions among infertile men in Kemerovo Region was 7.8%, comparable to other populations. Microdeletions in AZFс subregion, frequently correctable by assisted reproductive technology, were the predominant type. Conclusions. We recommend genotyping of the AZF locus to all male patients with primary infertility to exclude other causes of this disorder. This approach may also define whether assisted reproductive technology should be applied for infertility treatment in these patients, as in the case with microdeletions in AZFc subregion.
About the Authors
ALEXEY N. Volkov
Kemerovo State Medical University; Kemerovo Regional Clinical Hospital named after S.V. Belyaev
Russian Federation
Russian Federation
ELENA V. Tsurkan
Kemerovo Regional Clinical Hospital named after S.V. Belyaev
Russian Federation
Russian Federation
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Review
For citations:
Volkov A.N., Tsurkan E.V. PREVALENCE AND TYPES OF AZF MICRODELETIONS IN INFERTILE MEN. Fundamental and Clinical Medicine. 2018;3(4):6-11. (In Russ.)
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