Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women
https://doi.org/10.23946/2500-0764-2020-5-4-38-45
Abstract
Aim. To obtain a purified IgG preparation from the plasma of multiparous women and to evaluate its functional activity towards HLA-DR and HLA-G molecules.
Materials and Methods. IgG preparation was prepared by means of affinity chromatography using the DEAE Affi-Gel Blue system. The purity of the isolated preparation was assessed by immunoelectrophoresis. Functional activity of the IgG preparation against HLA-DR and HLA-G molecules was assessed in peripheral blood lymphocytes isolated from 14 apparently healthy men of reproductive age by flow cytometry.
Results. A 30 mL IgG fraction was obtained with a protein concentration of 4.3 g/l and a residual albumin less than 0.1 g/l. The protein concentration in the obtained fraction corresponded to the lower limit of IgG concentration in human serum. Immunoelectrophoresis showed that the IgG was the only antibody fraction in the chromatographic washout. Purified IgG fraction suppressed the expression of HLA-DR and HLA-G in donor lymphocytes.
Conclusion. The purified IgG fraction from multiparous women can have a significant preventive and therapeutic effect against immune disorders in the mother-embryo system and therefore might halt the development of congenital heart defects by blocking HLA-DR and HLA-G in the lymphocytes.
About the Authors
A. V. Shabaldin
Research Institute for Complex Issues of Cardiovascular Diseases;
Kemerovo State Medical University
Russian Federation
Russian Federation
Andrey V. Shabaldin, MD, DSc, Associate Professor, Department of Microbiology, Immunology and Virology; Leading Researcher
22a, Voroshilova Street, Kemerovo, 650056
6, Sosnovy Boulevard, Kemerovo, 650002
N. S. Deeva
Research Institute for Complex Issues of Cardiovascular Diseases
Russian Federation
Russian Federation
Nadezhda S. Deeva, MD, Junior Researcher, Laboratory of Cell and Tissue Engineering
6, Sosnovy Boulevard, Kemerovo, 650002
A. S. Sukhikh
Kemerovo State Medical University
Russian Federation
Russian Federation
Andrey S. Sukhikh, MD, PhD, Senior Researcher, Central Research Laboratory, Kemerovo State Medical University
22a, Voroshilova Street, Kemerovo, 650056
G. V. Vavin
Kemerovo State Medical University
Russian Federation
Russian Federation
Grigoriy V. Vavin, MD, PhD, Associate Professor, Department of Pathophysiology, Medical and Clinical Biochemistry, Kemerovo State Medical University
22a, Voroshilova Street, Kemerovo, 650056
References
1. Wu QY. [To promote the sustainable development of surgical treatment for congenital heart disease by innovation and practice]. Zhonghua Wai Ke Za Zhi. 2018;56(6):407-409. https://doi.org/10.3760/cma.j.issn.0529-5815.2018.06.003
2. Nagorneva SV, Prokhorova VS, Shelaeva EV, Khudovecova AM. The prevalence of congenital fetal anomalies for the past 5 years (2013-2017). Journal of Obstetrics and Women's Diseases. 2018;67(3):44-48. (In Russ.). https://doi.org/10.17816/ JOWD67344-48
3. Shabaldin AV, Shmulevich SA, Chistyakova GN, Remizova II, Lukoyanycheva EB, Gorshkova SV, Shabaldina EV. Peculiarities of allogenic interactions in the short-term culture of lymphocytes of spouses who have children with congenital heart diseases or early reproductive losses. Medical Immunology (Russia). 2019;21(2):279-292. (In Russ.). https://doi. org/10.15789/1563-0625-2019-2-279-292
4. Romanenko NA, Bessmel'cev SS, Chechetkin AV. Сorrection of patients’ immune status with human intravenous immunoglobulin. Kazan Medical Journal. 2017;5:775-783. (In Russ.).] https://doi. org/10.17750/KMJ2017-775
5. Nyborg KM, Kolte AM, Larsen EC, Christiansen OB. Immunomodulatory treatment with intravenous immunoglobulin and prednisone in patients with recurrent miscarriage and implantation failure after in vitro fertilization/intracytoplasmic sperm injection. Fertil Steril. 2014;102(6):1650-1655.e1. https://doi.org/10.1016/j. fertnstert.2014.08.029
6. Dubossarskaya ZM, Dubossarskaya YuA. Osnovnye voprosy immunologii reproduktsii. Medical Aspects of Women’s Health. 2010;4(31):15-21. (In Russ.).
7. Böyum A. Separation of leukocytes from blood and bone marrow. Introduction. Scand J Clin Lab Invest Suppl. 1968;97:7.
8. SHabaldin AV, Gorshkova SV, SHmulevich SA, Tsepokina AV, Vavin GV, Lukoyanycheva EB, SHabaldina EV, inventors; A method of pregravid prediction of the risk of sporadic septal congenital heart defects without chromosomal diseases in subsequent generations. Patent RUS US №2709610, C1. 19.12.2019. Available at: https:// yandex.ru/patents/doc/RU2709610C1_20191219 (In Russ.).
Review
For citations:
Shabaldin A.V., Deeva N.S., Sukhikh A.S., Vavin G.V. Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women. Fundamental and Clinical Medicine. 2020;5(4):38-45. (In Russ.) https://doi.org/10.23946/2500-0764-2020-5-4-38-45
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