Endothelial cells of arterial and venous blood in patients with various forms of coronary heart disease as a possible indicator of sustainability the morphofunctional system of the conduit artery

Aim. To conduct an assessment of the quantity, qualitative composition, and proliferative potential of blood ECs taken from the coronary artery (CA), internal mammary artery (IMA), radial artery (RA), and venous bed in patients with ASC and IHD.

Materials and methods. The study examined eight biological blood samples obtained from two male patients: a 72-year-old with ACS and a 63-year-old with stable IHD. Each patient had 20 ml of venous blood obtained from the cubital vein and 20 ml of arterial blood obtained from the internal thoracic, radial, and coronary arteries. The mononuclear fraction was isolated from the blood, the cells were counted, cultured in vitro.

Results. During 26 days of cultivation, cells in the vials were counted. The highest proliferative activity of ECFC was observed in a patient with ACS: the doubling time of the population was 2.17 days for cells isolated from blood of CA and 2.38 days for cells isolated from blood of IMA. In a patient with chronic IHD, the doubling time of ECFC cells obtained from venous blood was higher – 2.54 days. The highest concentration of ECFC was detected in a patient with ACS in the blood of the infarct-related artery.

Conclusion. An analysis of endothelial cells isolated from the blood of CA and conductive vessels in CS revealed differences depending on the form of IHD. In patients with ACS, similarities in the characteristics and growth of CA and IMA ECs were noted, which may reflect the unidirectionality of reparative processes and increased stability of the MFS of conduit arteries when using these autoarterial grafts.

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