PROINFLAMMATORY CYTOKINES, LACTOFERRIN, AND Α2-MACROGLOBULIN IN FOLLIC-ULAR FLUID AND SERUM OF WOMEN WITH UTERINE INFERTILITY AT THE START OF IN VITRO FERTILIZATION PROGRAM

Aim. To investigate the levels of proinflammatory cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, interferon (IFN)-γ), lactoferrin (LF), and α2-macroglobulin (α2-MG) in serum and follicular fluid of women with uterine infertility at the start of in vitro fertilization (IVF) program. Materials and Мethods. We collected 100 samples of follicular fluid and serum from women with uterine infertility following measurement of TNF-α, IL-6, IL-8, IFN-γ, and LF by enzyme-linked immunosorbent assay and α2-MG by rocket immunoelectrophoresis. Out of 100, 38 patients suffered from chronic endometritis, 25 were diagnosed with adenomyosis, and 37 women had primary tubal infertility. Results. No statistically significant differences were found between the patients with chronic endometritis and primary tubal infertility. Furthermore, there were no significant differences in serum cytokine, LF, or α2-MG levels between the patients with adenomyosis and primary tubal infertility. Strikingly, we detected higher serum levels of TNF-α and LF and reduced level of α2-MG in women with a negative IVF outcome. Follicular fluid of women with adenomyosis was characterized by an increased level of IFN-γ, which was also associated with the negative outcome of IVF. Conclusion. Imbalance of TNF-α, LF, and α2-MG in follicular fluid and serum of women with uterine infertility is associated with negative IVF outcome.

экстракорпоральное оплодотворение, беременность, хронический эндометрит, аденомиоз, цитокины, in vitro fertilization, pregnancy, chronic endometritis, adenomyosis, cytokines

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